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1860797230528593920
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2018-07-30 06:07:58
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| format |
Restricted Document
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11875
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UniSZA
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6175-01-FH02-FBIM-18-18241.pdf
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Word
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11875
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11875 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11875 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 8 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in Word 2018-07-30 06:07:58 6175-01-FH02-FBIM-18-18241.pdf UniSZA Private Access Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood Journal of Agrobiotechnology Vibrio cholerae is important water borne human pathogen that associated with cholera disease. The goal of this study was to determine the prevalence and antimicrobial resistance of V. cholerae found in seafood. Samples of prawn, cockles and squid samples bought from Kuala Terengganu and Besut markets. The detection of V. cholerae in the samples was done using Polymerase Chain Reaction (PCR) assay and plating method. V. cholerae isolates were subjected to six antibiotics susceptibility test using Kirby Bauer method. In Besut district, V. cholerae was found in cockles (33%), squid (38%) and prawn (44%) in supermarket. For wet market, V. cholerae only found in squid (18%) and no detection from prawn samples. For samples collected from Kuala Terengganu supermarket, the prevalence of V. cholera in cockle was at 29%, in squid at 56% and prawn at 75%. For samples collected from wet market, V. cholerae only present in squid at 18% and no detection in prawn samples. A total of 30 V. cholerae isolates were subjected to antibiotic testing. The isolates were completely resistance towards penicillin (100%), vancomycin (80%), tetracyline (23%), gentamycin (7%), norfloxacin (3%) and ciprofloxacin (3%). This study concluded prawn, cockle and squid samples from both district were contaminated with V. cholerae. Thus, it might pose risks to consumer if undercooked contaminated seafood were consumed. 9 1 50-57
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| spellingShingle |
Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood
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| summary |
Vibrio cholerae is important water borne human pathogen that associated with cholera disease. The goal of this study was to determine the prevalence and antimicrobial resistance of V. cholerae found in seafood. Samples of prawn, cockles and squid samples bought from Kuala Terengganu and Besut markets. The detection of V. cholerae in the samples was done using Polymerase Chain Reaction (PCR) assay and plating method. V. cholerae isolates were subjected to six antibiotics susceptibility test using Kirby Bauer method. In Besut district, V. cholerae was found in cockles (33%), squid (38%) and prawn (44%) in supermarket. For wet market, V. cholerae only found in squid (18%) and no detection from prawn samples. For samples collected from Kuala Terengganu supermarket, the prevalence of V. cholera in cockle was at 29%, in squid at 56% and prawn at 75%. For samples collected from wet market, V. cholerae only present in squid at 18% and no detection in prawn samples. A total of 30 V. cholerae isolates were subjected to antibiotic testing. The isolates were completely resistance towards penicillin (100%), vancomycin (80%), tetracyline (23%), gentamycin (7%), norfloxacin (3%) and ciprofloxacin (3%). This study concluded prawn, cockle and squid samples from both district were contaminated with V. cholerae. Thus, it might pose risks to consumer if undercooked contaminated seafood were consumed.
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| title |
Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood
|
| title_full |
Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood
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| title_fullStr |
Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood
|
| title_full_unstemmed |
Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood
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| title_short |
Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood
|
| title_sort |
detection, isolation and antibiotic testing of vibrio cholerae in seafood
|