In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin
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| internalnotes | 1. Bogardus C, et al.(1984) Effects of physical training and diet therapy on carbohydrate metabolism in patients with glucose intolerance and non-insulin dependent diabetes mellitus. Diabetes, 33: 311-318. 2. Tuomilehto J, et al. (2001) Prevention of type 2 diabetic mellitus by changes in lifestyle among subjects with impaired glucose tolerance. N. Eng. J. Med., 344(18): 1343-1350. 3. Fiocco AJ, et al. (2013) The effects of an exercise and lifestyle intervention program on cardiovascular, metabolic factors and cognitive performance in middle-aged adults with type II diabetes: A pilot study. Can. J. Diab., 37(4): 214-219. 4. King H, Aubert RE and Herman WH (1998) Global burden of diabetes, 1995-2025. Prevalence, numerical estimates and projections. Diab. Care, 21: 1414-1431. 5. Shaw JE, Sicree RA and Zimmet PL (2010) Global estimates of the prevalence of diabetes for 2010 and 2030. Diab. Res. Clin. Prac., 87(1): 4-14. 6. Ademiluyi AO and Oboh G (2013) Soybean phenolic-rich extracts inhibit key-enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin I converting enzyme) in vitro. Exp. Toxicol. Pathol., 65(3): 305-309. 7. Bahadoran Z, Mirmiran P and Azizi F (2013) Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. J. Diab. Metab. Disorders, 12: 43. 8. El-Abhar HS and Schaalan MF (2014) Phytotherapy in diabetes: review of potential mechanistic perspectives. World J. Diab., 5(2): 176-197. 9. Boden G and Zhang M (2006) Recent findings concerning thiazolidinediones in the treatment of diabetes. Expert Opinion Invest. Drugs, 15(3): 243-250. 10. McClatchey W (2002) From Polynesian healers to health food stores: changing perspectives of Morinda citrifolia (Rubiaceae). Integrat. Cancer Ther., 1(2), 110-120. 11. Wang, MY, et al. (2002) Morinda citrifolia (Noni): a literature review and recent advances in Noni research. Acta Pharmacol. Sinica, 23(12): 1127-1141. 12. Akihisa T, et al. (2007) Anti-inflammatory and potential cancer chemopreventive constituents of the fruits of Morinda citrifolia (Noni). J. Nat. Prod., 70(5): 754-757. 13. Deng S, et al. (2007) Lipoxygenase inhibitory constituents of the fruits of noni (Morinda citrifolia) collected in Tahiti. J. Nat. Prod., 70(5), 859-862. 14. Potterat O, et al. (2007). Identification of TLC markers and quantification by HPLC-MS of various constituents in noni fruit powder and commercial noni-derived products. J. Agric. Food Chem., 55(18), 7489-7494. 15. Mahattanadul S, et al. (2011) Effects of Morinda citrifolia aqueous fruit extract and its biomarker scopoletin on reflux esophagitis and gastric ulcer in rats. J. Ethnopharmac., 134(2), 243-250. 16. Rao USM and Subramaniam S (2009) Biochemical evaluation of antihyperglycemic and antioxidative effects of Morinda citrifolia fruit extract studied in streptozotocin-induced diabetic rats. Med. Chem. Res., 18(6): 433-446. 17. Subramaniam SP and Rao USM (2010) Amelioration of diabetic dyslipidemia by Morinda citrifolia fruits on streptozotocin induced diabetic rats. J. Pharm. Res., 3(4): 843-848. 18. Asfari M, et al. (1992) Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines. Endocrinology, 130(1): 167-178. 19. Tandrasasmita OM, et al.(2011) Glucose-lowering effect of DLBS3233 is mediated through phosphorylation of tyrosine and upregulation of PPARγ and GLUT4 expression. Int. J. Gen. Med., 4: 345. 20. Apostolidis E, Kwon, YI, and Shetty K (2007) Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innov. Food Sci. Emerging Technol., 8(1), 46-54. 21. Kazeem M I, Adamson JO and Ogunwande, IA (2013) Modes of inhibition of α-amylase and α-glucosidase by aqueous extract of Morinda lucida Benth leaf. Bio.Med. Res. Internat., 2013.Article ID 527570. 22. Prabhakar PK and Doble M (2011) Effects of natural products on commercial oral antidiabetic drugs in enhancing 2-deoxyglucose uptake by 3T3-L1 adipocytes. Ther. Adv. Endocrinol. Metab., 2(3): 113-114. 23. Ha DT, et al. (2010) Adlay seed extract (Coix lachryma-jobi L.) decreased adipocyte differentiation and increased glucose uptake in 3T3-L1 cells. J. Med. Food, 13(6): 1331-1339. 24. Shen Y, et al.(2010) Verification of antidiabetic effects of Cinnamomun zeylanicum using insulin-uncontrolled type 1 antidiabetic rats and cultured adipocytes. Biosci. Biotechnol. Biochem., 74 (12): 2418-2425. 25. Shen Y, et al. (2014) Cinnamon extract enhances glucose uptake in 3T3-L1 adipocytes and C2C12 myocytes by inducing LKB1-AMP-activated protein kinase signaling. PLoS ONE, 9(2): e 87894. 26. Noipha K, et al.(2008) In vitro glucose uptake activity of Tinospora crispa in skeletal muscle cells. Asian Biomed., 2(5): 415-420. 27. Yang MH, et al.(2013) Constituents from Terminalia species increase PPARα and PPARγ levels and stimulate glucose uptake without enhancing adipose differentiation. J. Ethnopharmacol., 149: 490-498. 28. Chen QC, et al. (2010) Flavonoids and isoflavonoids from Sophorae flos improve glucose uptake. Planta Medica, 76(1): 79-81. 29. Jin MN, et al.(2013) Flavonoids from Tetrastigma obtectum enhancing glucose consumption in insulin-resistance HepG2 cells via activating AMPK. Fitoterapia, 90: 240-246. 30. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth.,65(1): 55-63. 31. Mamidi MK, et al. (2012) Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications. J. Translat. Med., 10: 229. 32. Vongsak B, et al. (2013) maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by appropriate extraction methods. Indust. Crop Prod., 44: 566-571. 33. Nguyen PH, et al.(2013) Protein tyrosine phosphatase 1B (PTP1B) inhibitors from Morinda citrifolia (Noni) and their insulin mimetic activity. J. Nat. Prod., 76(11): 2080-2087. 34. Ismail TSES and Deshmukh SA (2012) Comparative study of effect of alpha glucosidase inhibitors-miglitol, acarbose and voglibose on postprandial hyperglycemia and glycosylated hemoglobin in type-2 diabetes mellitus. Int. J. Pharm. Bio. Sci., 3(3): 337-343. 35. Nickavar B and Amin G (2011) Enzyme assay guided isolation of an α-amylase inhibitor flavonoid from Vaccinium arctostaphylos leaves. Iranian J. Pharmac. Res., 10(4): 849-853. 36. Tadera K, et al.(2006) Inhibition of α-glucosidase and α-amylase by flavonoids. J. Nutr. Sci. Vitaminol., 52(2): 149-153. 37. Li YQ, et al. (2009) Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of α-glucosidase. J. Agric. Food Chem., 57(24): 11463-11468. 38. Kumar S, Kumar V and Prakash O (2013) Enzymes inhibition and antidiabetic effect of isolated constituents from Dillenia indica. BioMed Res. Int., Article ID 382063. 39. Hong HC, et al. (2013) Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea. Chin. Med., 8: 19. 40. Tan C, et al. (2013) Yeast α-glucosidase inhibitory phenolic compounds isolated from Gynura medica leaf. Int. J. Mol. Sci., 14(2): 2551-2558. |
| originalfilename | 5956-01-FH02-FBIM-15-02846.jpg |
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| spelling | 11688 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11688 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 1412 786 24 24 2015-04-20 15:14:39 1412x786 5956-01-FH02-FBIM-15-02846.jpg UniSZA Private Access In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin Research Journal of Pharmacy and Technology Diabetes mellitus is a metabolic disorder and management of blood glucose level is an important strategy in the control of the disease and complications associated with it. Therefore, components that cause uptake of glucose from the bloodstream and inhibitors of carbohydrate hydrolyzing enzymes can be useful in treatment of diabetes and medicinal plants are often used to achieve this aim. Morinda citrifolia fruit (MCF) is used in various countries for treatment of diabetes and the purpose of this study was to investigate the effect of MCF extract and its biomarker scopoletin on glucose uptake in HepG2 cells as well as its inhibitory effect on α-amylase and α-glucosidase. The safe doses for MCF extract and scopoletin were at 1 mg/ml and 0.2 μM, respectively as assessed by MTT assays and these were used for the assays. The extract had glucose uptake of 59.5% which was comparable to the standard metformin whereas the value for scopoletin was 30.6%. The extract had mild inhibitory activity on α-amylase and α-glucosidase with percentage of inhibition at 43.5% and 57%. The biomarker scopoletin showed lower activities at 23.9% and 35.7% for α-amylase and α-glucosidase respectively. Hence, these three activities may possibly be the mechanisms for MCF to exert its antidiabetic activity. 8 2 189-193 1. Bogardus C, et al.(1984) Effects of physical training and diet therapy on carbohydrate metabolism in patients with glucose intolerance and non-insulin dependent diabetes mellitus. Diabetes, 33: 311-318. 2. Tuomilehto J, et al. (2001) Prevention of type 2 diabetic mellitus by changes in lifestyle among subjects with impaired glucose tolerance. N. Eng. J. Med., 344(18): 1343-1350. 3. Fiocco AJ, et al. (2013) The effects of an exercise and lifestyle intervention program on cardiovascular, metabolic factors and cognitive performance in middle-aged adults with type II diabetes: A pilot study. Can. J. Diab., 37(4): 214-219. 4. King H, Aubert RE and Herman WH (1998) Global burden of diabetes, 1995-2025. Prevalence, numerical estimates and projections. Diab. Care, 21: 1414-1431. 5. Shaw JE, Sicree RA and Zimmet PL (2010) Global estimates of the prevalence of diabetes for 2010 and 2030. Diab. Res. Clin. Prac., 87(1): 4-14. 6. Ademiluyi AO and Oboh G (2013) Soybean phenolic-rich extracts inhibit key-enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin I converting enzyme) in vitro. Exp. Toxicol. Pathol., 65(3): 305-309. 7. Bahadoran Z, Mirmiran P and Azizi F (2013) Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. J. Diab. Metab. Disorders, 12: 43. 8. El-Abhar HS and Schaalan MF (2014) Phytotherapy in diabetes: review of potential mechanistic perspectives. World J. Diab., 5(2): 176-197. 9. Boden G and Zhang M (2006) Recent findings concerning thiazolidinediones in the treatment of diabetes. Expert Opinion Invest. Drugs, 15(3): 243-250. 10. McClatchey W (2002) From Polynesian healers to health food stores: changing perspectives of Morinda citrifolia (Rubiaceae). Integrat. Cancer Ther., 1(2), 110-120. 11. Wang, MY, et al. (2002) Morinda citrifolia (Noni): a literature review and recent advances in Noni research. Acta Pharmacol. Sinica, 23(12): 1127-1141. 12. Akihisa T, et al. (2007) Anti-inflammatory and potential cancer chemopreventive constituents of the fruits of Morinda citrifolia (Noni). J. Nat. Prod., 70(5): 754-757. 13. Deng S, et al. (2007) Lipoxygenase inhibitory constituents of the fruits of noni (Morinda citrifolia) collected in Tahiti. J. Nat. Prod., 70(5), 859-862. 14. Potterat O, et al. (2007). Identification of TLC markers and quantification by HPLC-MS of various constituents in noni fruit powder and commercial noni-derived products. J. Agric. Food Chem., 55(18), 7489-7494. 15. Mahattanadul S, et al. (2011) Effects of Morinda citrifolia aqueous fruit extract and its biomarker scopoletin on reflux esophagitis and gastric ulcer in rats. J. Ethnopharmac., 134(2), 243-250. 16. Rao USM and Subramaniam S (2009) Biochemical evaluation of antihyperglycemic and antioxidative effects of Morinda citrifolia fruit extract studied in streptozotocin-induced diabetic rats. Med. Chem. Res., 18(6): 433-446. 17. Subramaniam SP and Rao USM (2010) Amelioration of diabetic dyslipidemia by Morinda citrifolia fruits on streptozotocin induced diabetic rats. J. Pharm. Res., 3(4): 843-848. 18. Asfari M, et al. (1992) Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines. Endocrinology, 130(1): 167-178. 19. Tandrasasmita OM, et al.(2011) Glucose-lowering effect of DLBS3233 is mediated through phosphorylation of tyrosine and upregulation of PPARγ and GLUT4 expression. Int. J. Gen. Med., 4: 345. 20. Apostolidis E, Kwon, YI, and Shetty K (2007) Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innov. Food Sci. Emerging Technol., 8(1), 46-54. 21. Kazeem M I, Adamson JO and Ogunwande, IA (2013) Modes of inhibition of α-amylase and α-glucosidase by aqueous extract of Morinda lucida Benth leaf. Bio.Med. Res. Internat., 2013.Article ID 527570. 22. Prabhakar PK and Doble M (2011) Effects of natural products on commercial oral antidiabetic drugs in enhancing 2-deoxyglucose uptake by 3T3-L1 adipocytes. Ther. Adv. Endocrinol. Metab., 2(3): 113-114. 23. Ha DT, et al. (2010) Adlay seed extract (Coix lachryma-jobi L.) decreased adipocyte differentiation and increased glucose uptake in 3T3-L1 cells. J. Med. Food, 13(6): 1331-1339. 24. Shen Y, et al.(2010) Verification of antidiabetic effects of Cinnamomun zeylanicum using insulin-uncontrolled type 1 antidiabetic rats and cultured adipocytes. Biosci. Biotechnol. Biochem., 74 (12): 2418-2425. 25. Shen Y, et al. (2014) Cinnamon extract enhances glucose uptake in 3T3-L1 adipocytes and C2C12 myocytes by inducing LKB1-AMP-activated protein kinase signaling. PLoS ONE, 9(2): e 87894. 26. Noipha K, et al.(2008) In vitro glucose uptake activity of Tinospora crispa in skeletal muscle cells. Asian Biomed., 2(5): 415-420. 27. Yang MH, et al.(2013) Constituents from Terminalia species increase PPARα and PPARγ levels and stimulate glucose uptake without enhancing adipose differentiation. J. Ethnopharmacol., 149: 490-498. 28. Chen QC, et al. (2010) Flavonoids and isoflavonoids from Sophorae flos improve glucose uptake. Planta Medica, 76(1): 79-81. 29. Jin MN, et al.(2013) Flavonoids from Tetrastigma obtectum enhancing glucose consumption in insulin-resistance HepG2 cells via activating AMPK. Fitoterapia, 90: 240-246. 30. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth.,65(1): 55-63. 31. Mamidi MK, et al. (2012) Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications. J. Translat. Med., 10: 229. 32. Vongsak B, et al. (2013) maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by appropriate extraction methods. Indust. Crop Prod., 44: 566-571. 33. Nguyen PH, et al.(2013) Protein tyrosine phosphatase 1B (PTP1B) inhibitors from Morinda citrifolia (Noni) and their insulin mimetic activity. J. Nat. Prod., 76(11): 2080-2087. 34. Ismail TSES and Deshmukh SA (2012) Comparative study of effect of alpha glucosidase inhibitors-miglitol, acarbose and voglibose on postprandial hyperglycemia and glycosylated hemoglobin in type-2 diabetes mellitus. Int. J. Pharm. Bio. Sci., 3(3): 337-343. 35. Nickavar B and Amin G (2011) Enzyme assay guided isolation of an α-amylase inhibitor flavonoid from Vaccinium arctostaphylos leaves. Iranian J. Pharmac. Res., 10(4): 849-853. 36. Tadera K, et al.(2006) Inhibition of α-glucosidase and α-amylase by flavonoids. J. Nutr. Sci. Vitaminol., 52(2): 149-153. 37. Li YQ, et al. (2009) Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of α-glucosidase. J. Agric. Food Chem., 57(24): 11463-11468. 38. Kumar S, Kumar V and Prakash O (2013) Enzymes inhibition and antidiabetic effect of isolated constituents from Dillenia indica. BioMed Res. Int., Article ID 382063. 39. Hong HC, et al. (2013) Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea. Chin. Med., 8: 19. 40. Tan C, et al. (2013) Yeast α-glucosidase inhibitory phenolic compounds isolated from Gynura medica leaf. Int. J. Mol. Sci., 14(2): 2551-2558. |
| spellingShingle | In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin |
| summary | Diabetes mellitus is a metabolic disorder and management of blood glucose level is an important strategy in the control of the disease and complications associated with it. Therefore, components that cause uptake of glucose from the bloodstream and inhibitors of carbohydrate hydrolyzing enzymes can be useful in treatment of diabetes and medicinal plants are often used to achieve this aim. Morinda citrifolia fruit (MCF) is used in various countries for treatment of diabetes and the purpose of this study was to investigate the effect of MCF extract and its biomarker scopoletin on glucose uptake in HepG2 cells as well as its inhibitory effect on α-amylase and α-glucosidase. The safe doses for MCF extract and scopoletin were at 1 mg/ml and 0.2 μM, respectively as assessed by MTT assays and these were used for the assays. The extract had glucose uptake of 59.5% which was comparable to the standard metformin whereas the value for scopoletin was 30.6%. The extract had mild inhibitory activity on α-amylase and α-glucosidase with percentage of inhibition at 43.5% and 57%. The biomarker scopoletin showed lower activities at 23.9% and 35.7% for α-amylase and α-glucosidase respectively. Hence, these three activities may possibly be the mechanisms for MCF to exert its antidiabetic activity. |
| title | In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin |
| title_full | In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin |
| title_fullStr | In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin |
| title_full_unstemmed | In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin |
| title_short | In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin |
| title_sort | in vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of morinda citrifolia fruit and scopoletin |