Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis
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| internalnotes | 1. Ackermann G, Rodloff A, C. Drugs of the 21st century: telithromycin (HMR 3647)—the first ketolide. J Antimicrob Chemoth. 51, 2003, 497–511. 2. Ahmad SJ, Sudi S, Sidek HM, Basri DF, Zin NM, Anti-MRSA activity and Optimal Culture Condition of Streptomyces sp. isolate, SUK 25. (Article in Press), Jundishapur J Microbiol. 2013. 3. Basri DF, Jaffar N, Zin NM, Santhana Raj L, Electron microscope study of gall extract from Quercus infectoria in combination with vancomycin against MRSA using post-antibiotic effect determination. Int J Pharmacol. 9(2), 2013, 150-156. 4. Berdy J, Bioactive microbial metabolites. J Antibiot. 58, 2005, 1–26. 5. Bro¨tz H, Sahl H, New insights into the mechanism of action of Lantibiotics: diverse biological effects by binding to the same molecular target. J Antimicrob Chemoth. 46, 2000, 1–6. 6. Chen CZ, Cooper SL, Interactions between dendrimer biocides and bacterial membranes. Biomaterials, 23, 2002, 3359–3368. 7. CLSI, Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline, CLSI document NCCLS document M26-A (ISBN 1-56238-384-1). NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087 USA, 1999. 8. CLSI Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard— Eighth Edition. CLSI document M07-A8 (ISBN 1-56238-689-1). Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2009. 9. Cordwell SJ, Larsen MR, Cole RT, Walsh BJ, Comparative proteomics of Staphylococcus aureus and the response of methicillin-resistant and methicillin-sensitive strains to Triton X-100, Microbiol. 148, 2002, 2765–2781. 10. Credito KL, Ednie LM, Appelbaum PC, Comparative Antianaerobic Activities of Doripenem Determined by MIC and Time-Kill Analysis, Antimicrob Agents. 52(1), 2008, 365– 373. 11. Devi KP, Nisha SA, Sakthivel R, Pandian SK, Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane, J Ethnopharmacol. 130, 2010, 107–115. 12. Ezra D, Castillo UF, Strobel GA, Hess WM, Porter H, Jensen JB, Condron MAM, Teplow DB, Sears J, Maranta M, Hunter M, Weber B, Yaver D, Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp. Microbiol. 150, 2004, 785– 793. 13. Fujimoto DF, Bayles KW, Opposing Roles of the Staphylococcus aureus Virulence Regulators, Agr and Sar in Triton X-100-and Penicillin-Induced Autolysis, J Bacteriol. 180(14), 1998, 3724–3726. 14. Hanaki H, Kuwahara-Arai K, Boyle-Vavra S, Daum RS, Labischinski H, Hiramatsu K, Activated cell-wall synthesis is associated with vancomycin resistance in methicillin-resistant Staphylococcus aureus clinical strains Mu3 and Mu50, J Antimicrob Chemoth. 42, 1998, 199-209. 15. Hukari R, Helander IM, Vaara M, Chain length heterogeneity of lipopolysaccharide released from Salmonella typhimurium by ethylenediamineetetraacetic acid or polycations, Eur J Biochem. 154, 1986, 673-676. 16. Jeffres MN, Isakow W, Doherty JA, Micek ST, Kollef MH, A retrospective analysis of possible renal toxicity associated with vancomycin in patients with health care associated methicillin-resistant Staphylococcus aureus pneumonia. Clin Ther. 29(6), 2007, 1107-1115. 17. Li RC, Nix DE, Schentag JJ, New turbidimetric assay for quantitation of viable bacterial densities. Antimicrob Agents, 37(2), 1993, 371-374. 18. Li RC, Simultaneous pharmacodynamic analysis of the lag and bactericidal phases exhibited by beta-lactams against Escherichia coli. Antimicrob Agents, 40(10), 1996, 2306- 2310. 19. Liu H, Du Y, Wang X, Sun L, Chitosan kills bacteria through cell membrane damage, Int. J. Food Microbiol. 95, 2004, 147–155. 20. Mani N, Tobin P, Jayaswal RK, Isolation and Characterization of Autolysis-Defective Mutants of Staphylococcus aureus created by Tn917-lacZ Mutagenesis, J Bacteriol. 175(5), 1993, 1493-1499. 21. Malebo HM, Tanja W, Cal M, Swaleh SAM, Omolo MO, Hassanali A, Ali H, Sequin U, Hamburger M, Brun R, Ndiege IO, Antiplasmodial, anti-trypanosomal, anti-leishmanial and cytotoxicity activity of selected Tanzanian medicinal plants. Tanzania J Health Res 11(4), 2009, 226-234. 22. May J, Chan CH, King A, Willian L, French GL, Time-kill studies of tea tree oils on clinical isolates, J Antimicrob Chemoth. 45, 2000, 639-643. 23. Motta AS, Flores FS, Souto AA, Brandelli A, Antibacterial activity of a bacteriocin-like substance produced by Bacillus sp. P34 that targets the bacterial cell envelope, Antonie van Leeuwenhoek, 93, 2008, 275–284. 24. Mueller M, de la Pen˜a A, Derendorf H, Issues in Pharmacokinetics and Pharmacodynamics of Anti-Infective Agents: Kill Curves versus MIC, Antimicrob Agents, 48(2), 2004, 369–377. 25. Oonmetta-aree J, Suzuki T, Gasaluck P, Eumkeb G, Antimicrobial properties and action of galangal Alpinia galangal Linn. On Staphylococcus aureus, LWT-Food Sci Technol. 39(10), 2006, 1214-1220. 26. Raychaudhuri D, Chatterjee AN, Use of resistant mutants to study the interaction of Triton X-100 with Staphylococcus aureus. J Bacteriol. 164, 1985, 1337-1349. 27. Rogers HJ, Forsberg CW, Role of autolysins in the killing of bacteria by some bactericidal antibiotics. J Bacteriol. 108(3), 1971, 1235-1243. 28. Sianglum W, Srimanote P, Wonglumsom W, Kittiniyom K, Voravuthikunchai SP, Proteome analyses of cellular proteins in methicillin-resistant Staphylococcus aureus treated with rhodomyrtone, a novel antibiotic candidate. PloS one 6(2), 2011, 16628. 29. Strobel GA, Microbial gifts from rain forests. Can J Plant Pathol. 24, 2002, 14–20. 30. Sudha S, Masilamani, SM, Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4. Asian Pac J Trop Biomed. 2(10), 2012, 770-773. 31. Uehara T, Bernhardt TG, More than just lysins: peptidoglycan hydrolases tailor the cell wall. Current Opin Microbiol. 14, 2011, 698–703. 32. Zin NM, Sarmin NIM, Ghadin N, Basri DF, Sidik NM, Hess WM, Strobel GA Bioactive endophytic Streptomycetes from the Malay Peninsula, FEMS Microbiol Lett. 274, 2007, 83– 88. 33. Zin NM, Loi CS, Sarmin NM, Rosli AN, Cultivation-dependent characterization of endophytic actinomycetes. Res J Microbiol. 5(8), 2010, 717-724. |
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| spelling | 11542 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11542 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 1423 65 65 2015-02-15 10:30:50 799 1423x799 5795-01-FH02-FPSK-15-02549.jpg UniSZA Private Access Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis International Journal of Pharmaceutical Sciences Review and Research Bioactive compound from endophytic Streptomyces sp. has been claimed as a source of antibiotic. This study focused on the investigation of pharmacodynamic pattern and visualization of the mechanism of SUK 25 extracts against MRSA. The pharmacodynamic characteristic of the extract against MRSA 43300 was determined using time-kill assay. Then, the mode of action of the extracts was observed through biochemical assay and transmission electron microscopy. The SUK 25 extracts displayed bacteriostatic mode of action and concentration-dependent manner. The action of SUK 25 extracts against MRSA ATCC 43300 caused irregular shape of cells, which affected changes in Crystal Violet uptake by the cells. The release of UV absorbing materials and protein from the cells was caused by cell lysis. In conclusion, the action of the SUK 25 extracts against MRSA ATCC 43300 led to the internal change in cells, through which permeability of cells was altered by a decrease in the Crystal Violet uptake, shape of cell changes, and in turn brought about the lysis of cell. 30 1 11-17 1. Ackermann G, Rodloff A, C. Drugs of the 21st century: telithromycin (HMR 3647)—the first ketolide. J Antimicrob Chemoth. 51, 2003, 497–511. 2. Ahmad SJ, Sudi S, Sidek HM, Basri DF, Zin NM, Anti-MRSA activity and Optimal Culture Condition of Streptomyces sp. isolate, SUK 25. (Article in Press), Jundishapur J Microbiol. 2013. 3. Basri DF, Jaffar N, Zin NM, Santhana Raj L, Electron microscope study of gall extract from Quercus infectoria in combination with vancomycin against MRSA using post-antibiotic effect determination. Int J Pharmacol. 9(2), 2013, 150-156. 4. Berdy J, Bioactive microbial metabolites. J Antibiot. 58, 2005, 1–26. 5. Bro¨tz H, Sahl H, New insights into the mechanism of action of Lantibiotics: diverse biological effects by binding to the same molecular target. J Antimicrob Chemoth. 46, 2000, 1–6. 6. Chen CZ, Cooper SL, Interactions between dendrimer biocides and bacterial membranes. Biomaterials, 23, 2002, 3359–3368. 7. CLSI, Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline, CLSI document NCCLS document M26-A (ISBN 1-56238-384-1). NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087 USA, 1999. 8. CLSI Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard— Eighth Edition. CLSI document M07-A8 (ISBN 1-56238-689-1). Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2009. 9. Cordwell SJ, Larsen MR, Cole RT, Walsh BJ, Comparative proteomics of Staphylococcus aureus and the response of methicillin-resistant and methicillin-sensitive strains to Triton X-100, Microbiol. 148, 2002, 2765–2781. 10. Credito KL, Ednie LM, Appelbaum PC, Comparative Antianaerobic Activities of Doripenem Determined by MIC and Time-Kill Analysis, Antimicrob Agents. 52(1), 2008, 365– 373. 11. Devi KP, Nisha SA, Sakthivel R, Pandian SK, Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane, J Ethnopharmacol. 130, 2010, 107–115. 12. Ezra D, Castillo UF, Strobel GA, Hess WM, Porter H, Jensen JB, Condron MAM, Teplow DB, Sears J, Maranta M, Hunter M, Weber B, Yaver D, Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp. Microbiol. 150, 2004, 785– 793. 13. Fujimoto DF, Bayles KW, Opposing Roles of the Staphylococcus aureus Virulence Regulators, Agr and Sar in Triton X-100-and Penicillin-Induced Autolysis, J Bacteriol. 180(14), 1998, 3724–3726. 14. Hanaki H, Kuwahara-Arai K, Boyle-Vavra S, Daum RS, Labischinski H, Hiramatsu K, Activated cell-wall synthesis is associated with vancomycin resistance in methicillin-resistant Staphylococcus aureus clinical strains Mu3 and Mu50, J Antimicrob Chemoth. 42, 1998, 199-209. 15. Hukari R, Helander IM, Vaara M, Chain length heterogeneity of lipopolysaccharide released from Salmonella typhimurium by ethylenediamineetetraacetic acid or polycations, Eur J Biochem. 154, 1986, 673-676. 16. Jeffres MN, Isakow W, Doherty JA, Micek ST, Kollef MH, A retrospective analysis of possible renal toxicity associated with vancomycin in patients with health care associated methicillin-resistant Staphylococcus aureus pneumonia. Clin Ther. 29(6), 2007, 1107-1115. 17. Li RC, Nix DE, Schentag JJ, New turbidimetric assay for quantitation of viable bacterial densities. Antimicrob Agents, 37(2), 1993, 371-374. 18. Li RC, Simultaneous pharmacodynamic analysis of the lag and bactericidal phases exhibited by beta-lactams against Escherichia coli. Antimicrob Agents, 40(10), 1996, 2306- 2310. 19. Liu H, Du Y, Wang X, Sun L, Chitosan kills bacteria through cell membrane damage, Int. J. Food Microbiol. 95, 2004, 147–155. 20. Mani N, Tobin P, Jayaswal RK, Isolation and Characterization of Autolysis-Defective Mutants of Staphylococcus aureus created by Tn917-lacZ Mutagenesis, J Bacteriol. 175(5), 1993, 1493-1499. 21. Malebo HM, Tanja W, Cal M, Swaleh SAM, Omolo MO, Hassanali A, Ali H, Sequin U, Hamburger M, Brun R, Ndiege IO, Antiplasmodial, anti-trypanosomal, anti-leishmanial and cytotoxicity activity of selected Tanzanian medicinal plants. Tanzania J Health Res 11(4), 2009, 226-234. 22. May J, Chan CH, King A, Willian L, French GL, Time-kill studies of tea tree oils on clinical isolates, J Antimicrob Chemoth. 45, 2000, 639-643. 23. Motta AS, Flores FS, Souto AA, Brandelli A, Antibacterial activity of a bacteriocin-like substance produced by Bacillus sp. P34 that targets the bacterial cell envelope, Antonie van Leeuwenhoek, 93, 2008, 275–284. 24. Mueller M, de la Pen˜a A, Derendorf H, Issues in Pharmacokinetics and Pharmacodynamics of Anti-Infective Agents: Kill Curves versus MIC, Antimicrob Agents, 48(2), 2004, 369–377. 25. Oonmetta-aree J, Suzuki T, Gasaluck P, Eumkeb G, Antimicrobial properties and action of galangal Alpinia galangal Linn. On Staphylococcus aureus, LWT-Food Sci Technol. 39(10), 2006, 1214-1220. 26. Raychaudhuri D, Chatterjee AN, Use of resistant mutants to study the interaction of Triton X-100 with Staphylococcus aureus. J Bacteriol. 164, 1985, 1337-1349. 27. Rogers HJ, Forsberg CW, Role of autolysins in the killing of bacteria by some bactericidal antibiotics. J Bacteriol. 108(3), 1971, 1235-1243. 28. Sianglum W, Srimanote P, Wonglumsom W, Kittiniyom K, Voravuthikunchai SP, Proteome analyses of cellular proteins in methicillin-resistant Staphylococcus aureus treated with rhodomyrtone, a novel antibiotic candidate. PloS one 6(2), 2011, 16628. 29. Strobel GA, Microbial gifts from rain forests. Can J Plant Pathol. 24, 2002, 14–20. 30. Sudha S, Masilamani, SM, Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4. Asian Pac J Trop Biomed. 2(10), 2012, 770-773. 31. Uehara T, Bernhardt TG, More than just lysins: peptidoglycan hydrolases tailor the cell wall. Current Opin Microbiol. 14, 2011, 698–703. 32. Zin NM, Sarmin NIM, Ghadin N, Basri DF, Sidik NM, Hess WM, Strobel GA Bioactive endophytic Streptomycetes from the Malay Peninsula, FEMS Microbiol Lett. 274, 2007, 83– 88. 33. Zin NM, Loi CS, Sarmin NM, Rosli AN, Cultivation-dependent characterization of endophytic actinomycetes. Res J Microbiol. 5(8), 2010, 717-724. |
| spellingShingle | Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis |
| summary | Bioactive compound from endophytic Streptomyces sp. has been claimed as a source of antibiotic. This study focused on the investigation of pharmacodynamic pattern and visualization of the mechanism of SUK 25 extracts against MRSA. The pharmacodynamic characteristic of the extract against MRSA 43300 was determined using time-kill assay. Then, the mode of action of the extracts was observed through biochemical assay and transmission electron microscopy. The SUK 25 extracts displayed bacteriostatic mode of action and concentration-dependent manner. The action of SUK 25 extracts against MRSA ATCC 43300 caused irregular shape of cells, which affected changes in Crystal Violet uptake by the cells. The release of UV absorbing materials and protein from the cells was caused by cell lysis. In conclusion, the action of the SUK 25 extracts against MRSA ATCC 43300 led to the internal change in cells, through which permeability of cells was altered by a decrease in the Crystal Violet uptake, shape of cell changes, and in turn brought about the lysis of cell. |
| title | Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis |
| title_full | Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis |
| title_fullStr | Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis |
| title_full_unstemmed | Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis |
| title_short | Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis |
| title_sort | mode of action of endophytic streptomyces sp., suk 25 extracts against mrsa; microscopic, biochemical and time-kill analysis |