In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica

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spelling 11434 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11434 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 10 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in Adobe InDesign CS6 (Windows) 2017-11-01 21:09:50 xmp.id:D182A89605BFE711B488F9574DA76C6B 5677-01-FH02-FBIM-18-12610.pdf UniSZA Private Access In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica Pertanika Journal of Tropical Agricultural Science Azadirachta indica (Meliaceae; Neem tree) is a resourceful medicinal plant. Almost all parts of the plant are used in traditional and folklore medicine. This study was conducted to measure antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) in a crude extract of different parts of the neem tree. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging and Ferric-Reducing Antioxidant Power Assay (FRAP) were used to evaluate antioxidant activity, while total phenolic and total flavonoid compounds were measured using the Folin-Ciocalteu method and aluminium chloride (AlCl3 ) assay, respectively. Bark and leaf extract showed higher free radical scavenging activity, with IC50 value of 23.27 and 55.07 (μg/ml) respectively, while seed extract showed the lowest activity, with IC50 of 672.36 (μg/ml; (P<0.05). The bark extract revealed significantly higher antioxidant activity and phenolic content than the leaf and seed extract (P<0.05). Flavonoid content in leaf extract was found to be significantly higher (P<0.05) than in seed and bark extract; however, bark extract showed significantly lower flavonoid content than seed extract. These results suggest that the potency of Azadirachta indica makes it a good source of natural antioxidant compounds. 40 4 497-506
spellingShingle In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica
summary Azadirachta indica (Meliaceae; Neem tree) is a resourceful medicinal plant. Almost all parts of the plant are used in traditional and folklore medicine. This study was conducted to measure antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) in a crude extract of different parts of the neem tree. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging and Ferric-Reducing Antioxidant Power Assay (FRAP) were used to evaluate antioxidant activity, while total phenolic and total flavonoid compounds were measured using the Folin-Ciocalteu method and aluminium chloride (AlCl3 ) assay, respectively. Bark and leaf extract showed higher free radical scavenging activity, with IC50 value of 23.27 and 55.07 (μg/ml) respectively, while seed extract showed the lowest activity, with IC50 of 672.36 (μg/ml; (P<0.05). The bark extract revealed significantly higher antioxidant activity and phenolic content than the leaf and seed extract (P<0.05). Flavonoid content in leaf extract was found to be significantly higher (P<0.05) than in seed and bark extract; however, bark extract showed significantly lower flavonoid content than seed extract. These results suggest that the potency of Azadirachta indica makes it a good source of natural antioxidant compounds.
title In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica
title_full In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica
title_fullStr In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica
title_full_unstemmed In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica
title_short In-vitro antioxidant potential in leaf, stem and bark of Azadirachta indica
title_sort in-vitro antioxidant potential in leaf, stem and bark of azadirachta indica