Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract
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| date | 2014-12-10 16:23:10 |
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| internalnotes | 1. Zapata-Colindomethacinres JC, Zepeda-Go´mez S, Montan˜o-Loza A, Va´zquez-Ballesteros E, Villalobos JJ, Valdovinos-Andraca F. The association of Helicobacter pylori infection and nonsteroidal anti-inflammatory drugs in peptic ulcer disease. Can J Gastroenterol 2006; 20: 277–280. 2. Hoogerwerf WA, Pasricha PJ. Agents used for control of gastric acidity and treatment of peptic ulcers and gastroesophageal reflux disease. In: Harman JG, Limbird LE, Eds. Goodman & Gilman’s The Pharmacological Basis of Therapeutics. 10th edn, New York: McGraw-Hill; 2001; pp 1011-1012. 3. Zakaria ZA, Loo YW, Abdul Rahman NI, Abdul Ayub AH, Sulaiman MR, Hanan Kumar G. Antinociceptive, anti-inflammatory and antipyretic properties of Bauhinia purpurea leaves aqueous extract in experimental animals. Med Princ Pract 2007; 16: 443–449. 4. Yahya F, Mamat SS, Kamarolzaman MFF, Seyyedan A, Jakius KF, Mahmood ND, Shahril MS, Suhaili Z, Mohtarrudin N, Susanti D, Somchit MN, Teh LK, Salleh MZ, Zakaria ZA. Hepatoprotective activity of methanolic extract of leaves of Bauhinia purpurea in paracetamol-induced hepatic damage in rats. Evid Compliment Altern Med 2013; 2013: 1–10. 5. Institute for Laboratory Animal Research. Guide for the care and use of laboratory animals, 8th edn. Washington (DC): National Academies Press, 2011. 6. Zabidi Z, Wan Zainulddin WN, Mamat SS, Shamsahal Din S, Kamisan FH, Yahya F, Ismail NA, Rodzi R, Hussain H, Mohtarrudin N, Somchit MN, Zakaria ZA. Antiulcer activity of methanol extract of Melastoma malabathricum leaves in rats. Med Princ Pract 2012; 21: 501–503. 7. Gupta M, Mazumder UK, Manikandan L, Bhattacharya S, Senthilkumarm GP, Suresh R. Anti-ulcer activity of ethanol extract of Terminalia pallida Brandis in Swiss albino rats. J Ethnopharmacol 2005; 97: 405-408. 8. Shay H, Komarov SA, Fels SS, Meranze D, Gruenstein M, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 1945; 5: 43–61. 9. Abdul Hisam EE, Zakaria ZA, Mohtaruddin N, Rofiee MS, Hamid AH, Othman F. Antiulcer Activity of the Chloroform Extract of Bauhinia purpurea Leaf. Pharmaceut Biol 2012; 50: 1498–1507. 10. Corne SJ, Morrissey SM, Woods RJ. A Method for the quantitative estimation of gastric barrier mucous. J Physiol 1974; 242: 116P–117P. 11. Andreo MA, Ballesteros KVR, Hiruma-Lima CA, Machado da Rocha LR, Souza Brito, ARM, Vilegas W. Effect of Mouriri pusa extracts on experimentally indomethacin-induced gastric lesions in rodents: Role of endogenous sulfhydryls compounds and nitric oxide in gastroprotection. J Ethnopharmacol 2006; 107: 431-441. 12. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181: 1199–1200. 13. Liu F, Ooi VEC, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 1997; 60: 763-771. 14. Cao G, Prior RL. Measurement of oxygen radical absorbance capacity in biological samples. Methods Enzymol. 1999; 299: 50-62. 15. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965; 16: 144-158. 16. Nordmann R. Alcohol and antioxidant systems. Alcohol and Alcoholism. 1994; 29: 513-522. 17. Kitagawa H, Kurahashi K, Fujiwara M, Kohei H. Antiulcerogenic effect of a pyrido-benzodazepine derivative (L-S519) on experimental ulcers. Arzneim Forsch 1978; 28: 2122-2127. 18. Chandranath SI, Bastaki SM, Singh J. A comparative study on the activity of lansoprazole, omeprazole and PD-136450 on acidified ethanol- and indomethacin-induced gastric lesions in the rat. Clin Exp Pharmacol Physiol 2002; 29: 173–180. 19. Santin JR, Lemos M, Klein-Júnior LC, Machado ID, Costa P, de Oliveira AP, Tilia C, de Souza JP, de Sousa JP, Bastos JK, De Andrade SF. Gastroprotective activity of essential oil of the Syzygium aromaticum and its major component eugenol in different animal models. Naunyn Schmiedebergs Arch Pharmacol 2011; 383: 149-158. 20. Silva MI, Moura BA, Neto MR, Tomé AR, Rocha NF, de Carvalho AM, Macêdo DS, Vasconcelos SM, de Sousa DP, Viana GS, de Sousa FC. Gastroprotective activity of isopulegol on experimentally induced gastric lesions in mice: investigation of possible mechanisms of action. Naunyn Schmiedebergs Arch Pharmacol 2009; 380: 233-245. 21. Bruna P, Siqueira J, Menezes CT, Silva JP, de Sousa DP, Batista JS. Antiulcer effect of epoxy-carvone. Revista Brasileira de Farmacognosia Brazilian J Pharmacognosy 2012; 22(1): 144-149. 22. Maity S, Vedasiromoni JR, Ganguly DK. Role of glutathione in the antiulcer effect of hot water extract of black tea (Camellia sinensis). Jpn J Pharmacol. 1998; 78: 285-292. |
| originalfilename | 5577-01-FH02-FBIM-14-02095.jpg |
| person | UniSZA Unisza unisza |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11354 |
| spelling | 11354 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11354 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 29 29 797 1425 2014-12-10 16:23:10 1425x797 5577-01-FH02-FBIM-14-02095.jpg UniSZA Private Access Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract Tropical Journal of Pharmaceutical Research Purpose: To determine the gastroprotective activity and mechanisms of protection of the methanol extract of Bauhinia purpurea leaves (MEBP) using ethanol-induced gastric ulcer model. Methods: Male Sprague Dawley rats (n = 6) were administered orally with 10 % DMSO, 100 mg/kg ranitidine or MEBP (50, 250 and 500 mg / kg) daily for 7 consecutive days prior to subjection to the ethanol-induced gastric ulcer assay. The mechanisms of gastroprotection were determined based on: i) antisecretory activity via pylorus ligation assay; ii) the role of nitric oxide (NO) and sulfhydryl group via pre-treatment of MEBP-treated rats with the respective N-nitro-L-arginine methyl ester (L-NAME) or carbenoxolone (CBX) followed by the ethanol-induced assay; and iii) antioxidant activity using superoxide anion radical scavenging assay and, oxygen radical absorbance capacity (ORAC) assay. Ranitidine (100 mg / kg) was used as the reference drug. . Results: MEBP exhibited a significant (p < 0.05) and dose-dependent gastroprotective activity against ethanol-induced gastric ulcer with ulcer formation ranging between 0 and 74 % (indicated by decrease in ulcer area from 21.3 to 5.5 mm2). The macroscopic observation was in line with the microscopic findings and further supported by the histological scores suggesting the antiulcer potential of MEBP. MEBP also significantly (p < 0.05) reduced volume gastric juice, as well as its free and total acidity while increasing its pH. Moreover, this activity was significantly (p < 0.05) modulated in the presence of sulfhydryl group, but not NO. The extract also exhibited significant (p < 0.05) antioxidant activity. Conclusion: MEBP exerts gastroprotective activity partly via its antisecretory and antioxidant activities, as well as by modulation of sulfhydryl group, but not NO action. 13 11 1889-1898 1. Zapata-Colindomethacinres JC, Zepeda-Go´mez S, Montan˜o-Loza A, Va´zquez-Ballesteros E, Villalobos JJ, Valdovinos-Andraca F. The association of Helicobacter pylori infection and nonsteroidal anti-inflammatory drugs in peptic ulcer disease. Can J Gastroenterol 2006; 20: 277–280. 2. Hoogerwerf WA, Pasricha PJ. Agents used for control of gastric acidity and treatment of peptic ulcers and gastroesophageal reflux disease. In: Harman JG, Limbird LE, Eds. Goodman & Gilman’s The Pharmacological Basis of Therapeutics. 10th edn, New York: McGraw-Hill; 2001; pp 1011-1012. 3. Zakaria ZA, Loo YW, Abdul Rahman NI, Abdul Ayub AH, Sulaiman MR, Hanan Kumar G. Antinociceptive, anti-inflammatory and antipyretic properties of Bauhinia purpurea leaves aqueous extract in experimental animals. Med Princ Pract 2007; 16: 443–449. 4. Yahya F, Mamat SS, Kamarolzaman MFF, Seyyedan A, Jakius KF, Mahmood ND, Shahril MS, Suhaili Z, Mohtarrudin N, Susanti D, Somchit MN, Teh LK, Salleh MZ, Zakaria ZA. Hepatoprotective activity of methanolic extract of leaves of Bauhinia purpurea in paracetamol-induced hepatic damage in rats. Evid Compliment Altern Med 2013; 2013: 1–10. 5. Institute for Laboratory Animal Research. Guide for the care and use of laboratory animals, 8th edn. Washington (DC): National Academies Press, 2011. 6. Zabidi Z, Wan Zainulddin WN, Mamat SS, Shamsahal Din S, Kamisan FH, Yahya F, Ismail NA, Rodzi R, Hussain H, Mohtarrudin N, Somchit MN, Zakaria ZA. Antiulcer activity of methanol extract of Melastoma malabathricum leaves in rats. Med Princ Pract 2012; 21: 501–503. 7. Gupta M, Mazumder UK, Manikandan L, Bhattacharya S, Senthilkumarm GP, Suresh R. Anti-ulcer activity of ethanol extract of Terminalia pallida Brandis in Swiss albino rats. J Ethnopharmacol 2005; 97: 405-408. 8. Shay H, Komarov SA, Fels SS, Meranze D, Gruenstein M, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 1945; 5: 43–61. 9. Abdul Hisam EE, Zakaria ZA, Mohtaruddin N, Rofiee MS, Hamid AH, Othman F. Antiulcer Activity of the Chloroform Extract of Bauhinia purpurea Leaf. Pharmaceut Biol 2012; 50: 1498–1507. 10. Corne SJ, Morrissey SM, Woods RJ. A Method for the quantitative estimation of gastric barrier mucous. J Physiol 1974; 242: 116P–117P. 11. Andreo MA, Ballesteros KVR, Hiruma-Lima CA, Machado da Rocha LR, Souza Brito, ARM, Vilegas W. Effect of Mouriri pusa extracts on experimentally indomethacin-induced gastric lesions in rodents: Role of endogenous sulfhydryls compounds and nitric oxide in gastroprotection. J Ethnopharmacol 2006; 107: 431-441. 12. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181: 1199–1200. 13. Liu F, Ooi VEC, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 1997; 60: 763-771. 14. Cao G, Prior RL. Measurement of oxygen radical absorbance capacity in biological samples. Methods Enzymol. 1999; 299: 50-62. 15. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965; 16: 144-158. 16. Nordmann R. Alcohol and antioxidant systems. Alcohol and Alcoholism. 1994; 29: 513-522. 17. Kitagawa H, Kurahashi K, Fujiwara M, Kohei H. Antiulcerogenic effect of a pyrido-benzodazepine derivative (L-S519) on experimental ulcers. Arzneim Forsch 1978; 28: 2122-2127. 18. Chandranath SI, Bastaki SM, Singh J. A comparative study on the activity of lansoprazole, omeprazole and PD-136450 on acidified ethanol- and indomethacin-induced gastric lesions in the rat. Clin Exp Pharmacol Physiol 2002; 29: 173–180. 19. Santin JR, Lemos M, Klein-Júnior LC, Machado ID, Costa P, de Oliveira AP, Tilia C, de Souza JP, de Sousa JP, Bastos JK, De Andrade SF. Gastroprotective activity of essential oil of the Syzygium aromaticum and its major component eugenol in different animal models. Naunyn Schmiedebergs Arch Pharmacol 2011; 383: 149-158. 20. Silva MI, Moura BA, Neto MR, Tomé AR, Rocha NF, de Carvalho AM, Macêdo DS, Vasconcelos SM, de Sousa DP, Viana GS, de Sousa FC. Gastroprotective activity of isopulegol on experimentally induced gastric lesions in mice: investigation of possible mechanisms of action. Naunyn Schmiedebergs Arch Pharmacol 2009; 380: 233-245. 21. Bruna P, Siqueira J, Menezes CT, Silva JP, de Sousa DP, Batista JS. Antiulcer effect of epoxy-carvone. Revista Brasileira de Farmacognosia Brazilian J Pharmacognosy 2012; 22(1): 144-149. 22. Maity S, Vedasiromoni JR, Ganguly DK. Role of glutathione in the antiulcer effect of hot water extract of black tea (Camellia sinensis). Jpn J Pharmacol. 1998; 78: 285-292. |
| spellingShingle | Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract |
| summary | Purpose: To determine the gastroprotective activity and mechanisms of protection of the methanol extract of Bauhinia purpurea leaves (MEBP) using ethanol-induced gastric ulcer model. Methods: Male Sprague Dawley rats (n = 6) were administered orally with 10 % DMSO, 100 mg/kg ranitidine or MEBP (50, 250 and 500 mg / kg) daily for 7 consecutive days prior to subjection to the ethanol-induced gastric ulcer assay. The mechanisms of gastroprotection were determined based on: i) antisecretory activity via pylorus ligation assay; ii) the role of nitric oxide (NO) and sulfhydryl group via pre-treatment of MEBP-treated rats with the respective N-nitro-L-arginine methyl ester (L-NAME) or carbenoxolone (CBX) followed by the ethanol-induced assay; and iii) antioxidant activity using superoxide anion radical scavenging assay and, oxygen radical absorbance capacity (ORAC) assay. Ranitidine (100 mg / kg) was used as the reference drug. . Results: MEBP exhibited a significant (p < 0.05) and dose-dependent gastroprotective activity against ethanol-induced gastric ulcer with ulcer formation ranging between 0 and 74 % (indicated by decrease in ulcer area from 21.3 to 5.5 mm2). The macroscopic observation was in line with the microscopic findings and further supported by the histological scores suggesting the antiulcer potential of MEBP. MEBP also significantly (p < 0.05) reduced volume gastric juice, as well as its free and total acidity while increasing its pH. Moreover, this activity was significantly (p < 0.05) modulated in the presence of sulfhydryl group, but not NO. The extract also exhibited significant (p < 0.05) antioxidant activity. Conclusion: MEBP exerts gastroprotective activity partly via its antisecretory and antioxidant activities, as well as by modulation of sulfhydryl group, but not NO action. |
| title | Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract |
| title_full | Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract |
| title_fullStr | Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract |
| title_full_unstemmed | Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract |
| title_short | Gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract |
| title_sort | gastroprotective activity and mechanisms of action of bauhinia purpurea linn (leguminoseae) leaf methanol extract |