Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression
| Format: | Restricted Document |
|---|
| _version_ | 1860797085754851328 |
|---|---|
| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 |
| date | 2014-10-28 15:00:09 |
| format | Restricted Document |
| id | 11316 |
| institution | UniSZA |
| internalnotes | 1. Wild, S., Roglic, G., Green, A. et al. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004; 27: 5,1047-1053. doi:10.2337/diacare.27.5.1047 2. Singer, A.J., Clark, R.A.F. Cutaneous wound healing, mechanisms of disease. N Engl J Med 1999; 341: 10, 738-746. doi:10.1056/NEJM199909023411006 3. Boulton, A.J.M. The diabetic foot: a global view. Diabetes Metab Res Rev 2000; 16: Suppl 1, S2–S5. doi:10.1002/1520- 7560(200009/10)16:1+<::AIDDMRR105>3.0.CO;2-N 4. Reiber, G.E., Boyko, E.J., Smith, D.G. Lower extremity foot ulcers and amputations in diabetes. In: Harris, M.I., Stern, M.P. (eds). Diabetes in America. Bethesda. 1995. 5. Teoh, S.L., Latiff, A.A., Das, S. The effect of topical extract of Momordica charantia (bitter gourd) on wound healing in nondiabetic rats and in rats with diabetes induced by streptozotocin. Clin Exp Dermatol 2009; 34: 815–822. doi:10.1111/j.1365-2230.2008.03117.x 6. Prasad, V., Jain, V., Girish, D., Dorle, A.K. Wound-healing property of Momordica charantia L. fruit powder. J Herb Pharmacother 2006; 6: 3/4, 105-115. doi:10.1300/ J157v06n03_05 7. Krawinkel, M.B., Keding, G.B. Bitter gourd (Momordica charantia): A dietary approach to hyperglycemia. Nutr Rev 2006; 64: 7, 331–333. doi:10.1111/j.1753-4887.2006. tb00217.x 8. Shetty, A.K., Suresh, K.G., Sambaiah, K., Salimath, P.V. Effect of bitter gourd (Momordica charantia) on glycaemic status in streptozotocin induced diabetic rats. Plant Foods Hum Nutr 2005; 60, 109–112. doi:10.1007/s11130-005-6837-x 9. Sathishsekar, D., Subramanian, S. Antioxidant properties of Momordica Charantia (bitter gourd) seeds on streptozotocin induced diabetic rats. Asia Pac J Clin Nutr 2005; 14: 2, 153-158. 10. Chong-Kuei, L., Haw-Wen, C., Wen-Tzu, Y., Kai-Li, L. Suppressive effects of wild bittergourd (Momordica charantia Linn. var. abbreviata ser.) fruit extracts on inflammatory responses in RAW 264.7 macrophages. J Ethnopharmacol 2009; 122: 2, 227-233. doi:10.1016/j. jep.2009.01.028 11. Khanna, P., Jain, S.C., Panagariya, A., Dixit, V.P. Hypoglycemic activity of polypeptide-p from a plant source. J Nat Prod 1981; 44, 648-665. doi: 0.1021/np50018a002 12. Raman, A., Lau, C. Anti-diabetic properties and phytochemistry of Momordica charantia L. (Cucurbitaceae). Phytomedicine 1996; 2, 349-362. doi:10.1016/S0944-7113(96)80080-8 13. Sofowora, A. Medicinal plants and traditional medicine in Africa. John Wiley and Sons, 1982. 14. Rolandelli, R.H., Koruda, M.J., Settle, R.G., Rombeau, J.L. The effect of enteral feedings supplemented with pectin on the healing of colonic anastomoses in the rat. Surgery 1986; 99: 6, 703-707. 15. Morisaki, N., Watanabe, S., Tezuka, M. et al. Mechanism of angiogenic effects of saponin from ginseng Radix rubra in human umbilical vein endothelial cells. Br J Pharmacol 1995; 115: 7, 1188-1193. doi:10.1111/j.1476-5381.1995. tb15023.x 16. Wu, S.J., Ng, L.T. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) in Taiwan. LWT- Food Science and Technology 2008; 41, 323-330. doi:10.1016/j.lwt.2007.03.003 17. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem1976; 72, 123-125. doi:10.1016/0003-2697(76)90527-3 18. Sharma, S., Sharma, M.C., Kohli, D.V. Wound healing activity of the ether-chloroform extract of Momordica charantia fruits in rats. Dig J Nanomater Biostruct 2010; 5:1, 123-126. 19. Lobmann, R., Schultz ,G., Lehnert, H. Proteases and the diabetic foot syndrome: Mechanisms and therapeutic implications. Diabetes Care 2005; 28, 461–471. doi:10.2337/ diacare.28.2.461 20. Bitar, M.S., Labbad, Z.N. Transforming growth factor-β and insulin-like growth factor-1 in relation to diabetes-induced impairment of wound healing. J Surg Res 1996; 61: 1, 113-119. doi:10.1006/jsre.1996.0090 21. Montesano, R., Orci, L. Transforming growth factor β stimulates collagen-matrix contraction by fibroblasts: Implications for wound healing. Proc Natl Acad Sci USA 1988; 85, 4894-4897. 22. Beanes, S.R., Dang, C., Chia, S., Kang, T. Skin repair and scar formation: the central role of TGF-β. Expert Rev Mol Med 2003; 5: 8, 1-22. doi:http://dx.doi. org/10.1017/S1462399403005817 23. Mian, M., Beghè, F., Mian, E. Collagen as a pharmacological approach in wound healing. Int J Tissue React. 1992; 14, 1-9. 24. Shah, M., Foreman, D.M., Ferguson, M.W. Neutralisation of TGF-beta 1 and TGF-beta 2 or exogenous addition of TGF-beta 3 to cutaneous rat wounds reduces scarring. J Cell Sci 1995; 108, 985-1002. 25. Greenhalgh, D.G. The role of growth factors in wound healing. J Trauma 1996; 41, 159-167. 26. Braca, A., Siciliano, T., D'Arrigo, M., Germano, M.P. Chemical composition and antimicrobial activity of Momordica charantia seed essential oil. Fitoterapia 2008; 79, 123-125. doi:10.1016/j.fitote.2007.11.002 |
| originalfilename | 5533-01-FH02-FPSK-14-01575.jpg |
| person | UniSZA Unisza unisza |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11316 |
| spelling | 11316 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11316 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 787 1422 20 20 1422x787 2014-10-28 15:00:09 5533-01-FH02-FPSK-14-01575.jpg UniSZA Private Access Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression Journal of Wound Care Objective: Transforming growth factor-β (TGF-β) plays an important role in wound healing. Delayed wound healing is a consequence of diabetes, leading to high morbidity and poor quality of life. Momordica charantia (MC) fruit possesses anti-diabetic and wound healing properties. This study aimed to explore the changes in TGF-β expression in diabetic wounds treated with topical MC fruit extract. Methods: Fifty-six male Sprague-Dawley rats were divided into a normal control group and five diabetic groups of ten rats each. Intravenous streptozotocin (50mg/kg) was given to induce diabetes in the diabetic groups. Full thickness excision wounds were created on the thoracodorsal region of the animals, and these wounds were then treated with vehicle, MC powder, MC ointment and povidone ointment or ointment base for ten days. Wound healing was determined by the rate of wound closure, total protein content and TGF-β expression in the wounds, and histological observation. Results: Diabetic groups showed delayed wound closure rates compared to the control group. The wound closure rate in the MC ointment group was significantly faster than that of the untreated diabetic group (p<0.05). The MC ointment group also showed intense TGF-β expression and a high level of total protein content. Conclusion: MC ointment has a promising potential for use as an alternative topical medication for diabetic wounds. This work has shown that it accelerates wound healing in diabetic rats, and it is suggested here that this occurs by enhancing TGF-β expression. Further work is recommended to explore this effect. Declaration of interest: There is no conflict of interest. 23 8 400-407 1. Wild, S., Roglic, G., Green, A. et al. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004; 27: 5,1047-1053. doi:10.2337/diacare.27.5.1047 2. Singer, A.J., Clark, R.A.F. Cutaneous wound healing, mechanisms of disease. N Engl J Med 1999; 341: 10, 738-746. doi:10.1056/NEJM199909023411006 3. Boulton, A.J.M. The diabetic foot: a global view. Diabetes Metab Res Rev 2000; 16: Suppl 1, S2–S5. doi:10.1002/1520- 7560(200009/10)16:1+<::AIDDMRR105>3.0.CO;2-N 4. Reiber, G.E., Boyko, E.J., Smith, D.G. Lower extremity foot ulcers and amputations in diabetes. In: Harris, M.I., Stern, M.P. (eds). Diabetes in America. Bethesda. 1995. 5. Teoh, S.L., Latiff, A.A., Das, S. The effect of topical extract of Momordica charantia (bitter gourd) on wound healing in nondiabetic rats and in rats with diabetes induced by streptozotocin. Clin Exp Dermatol 2009; 34: 815–822. doi:10.1111/j.1365-2230.2008.03117.x 6. Prasad, V., Jain, V., Girish, D., Dorle, A.K. Wound-healing property of Momordica charantia L. fruit powder. J Herb Pharmacother 2006; 6: 3/4, 105-115. doi:10.1300/ J157v06n03_05 7. Krawinkel, M.B., Keding, G.B. Bitter gourd (Momordica charantia): A dietary approach to hyperglycemia. Nutr Rev 2006; 64: 7, 331–333. doi:10.1111/j.1753-4887.2006. tb00217.x 8. Shetty, A.K., Suresh, K.G., Sambaiah, K., Salimath, P.V. Effect of bitter gourd (Momordica charantia) on glycaemic status in streptozotocin induced diabetic rats. Plant Foods Hum Nutr 2005; 60, 109–112. doi:10.1007/s11130-005-6837-x 9. Sathishsekar, D., Subramanian, S. Antioxidant properties of Momordica Charantia (bitter gourd) seeds on streptozotocin induced diabetic rats. Asia Pac J Clin Nutr 2005; 14: 2, 153-158. 10. Chong-Kuei, L., Haw-Wen, C., Wen-Tzu, Y., Kai-Li, L. Suppressive effects of wild bittergourd (Momordica charantia Linn. var. abbreviata ser.) fruit extracts on inflammatory responses in RAW 264.7 macrophages. J Ethnopharmacol 2009; 122: 2, 227-233. doi:10.1016/j. jep.2009.01.028 11. Khanna, P., Jain, S.C., Panagariya, A., Dixit, V.P. Hypoglycemic activity of polypeptide-p from a plant source. J Nat Prod 1981; 44, 648-665. doi: 0.1021/np50018a002 12. Raman, A., Lau, C. Anti-diabetic properties and phytochemistry of Momordica charantia L. (Cucurbitaceae). Phytomedicine 1996; 2, 349-362. doi:10.1016/S0944-7113(96)80080-8 13. Sofowora, A. Medicinal plants and traditional medicine in Africa. John Wiley and Sons, 1982. 14. Rolandelli, R.H., Koruda, M.J., Settle, R.G., Rombeau, J.L. The effect of enteral feedings supplemented with pectin on the healing of colonic anastomoses in the rat. Surgery 1986; 99: 6, 703-707. 15. Morisaki, N., Watanabe, S., Tezuka, M. et al. Mechanism of angiogenic effects of saponin from ginseng Radix rubra in human umbilical vein endothelial cells. Br J Pharmacol 1995; 115: 7, 1188-1193. doi:10.1111/j.1476-5381.1995. tb15023.x 16. Wu, S.J., Ng, L.T. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) in Taiwan. LWT- Food Science and Technology 2008; 41, 323-330. doi:10.1016/j.lwt.2007.03.003 17. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem1976; 72, 123-125. doi:10.1016/0003-2697(76)90527-3 18. Sharma, S., Sharma, M.C., Kohli, D.V. Wound healing activity of the ether-chloroform extract of Momordica charantia fruits in rats. Dig J Nanomater Biostruct 2010; 5:1, 123-126. 19. Lobmann, R., Schultz ,G., Lehnert, H. Proteases and the diabetic foot syndrome: Mechanisms and therapeutic implications. Diabetes Care 2005; 28, 461–471. doi:10.2337/ diacare.28.2.461 20. Bitar, M.S., Labbad, Z.N. Transforming growth factor-β and insulin-like growth factor-1 in relation to diabetes-induced impairment of wound healing. J Surg Res 1996; 61: 1, 113-119. doi:10.1006/jsre.1996.0090 21. Montesano, R., Orci, L. Transforming growth factor β stimulates collagen-matrix contraction by fibroblasts: Implications for wound healing. Proc Natl Acad Sci USA 1988; 85, 4894-4897. 22. Beanes, S.R., Dang, C., Chia, S., Kang, T. Skin repair and scar formation: the central role of TGF-β. Expert Rev Mol Med 2003; 5: 8, 1-22. doi:http://dx.doi. org/10.1017/S1462399403005817 23. Mian, M., Beghè, F., Mian, E. Collagen as a pharmacological approach in wound healing. Int J Tissue React. 1992; 14, 1-9. 24. Shah, M., Foreman, D.M., Ferguson, M.W. Neutralisation of TGF-beta 1 and TGF-beta 2 or exogenous addition of TGF-beta 3 to cutaneous rat wounds reduces scarring. J Cell Sci 1995; 108, 985-1002. 25. Greenhalgh, D.G. The role of growth factors in wound healing. J Trauma 1996; 41, 159-167. 26. Braca, A., Siciliano, T., D'Arrigo, M., Germano, M.P. Chemical composition and antimicrobial activity of Momordica charantia seed essential oil. Fitoterapia 2008; 79, 123-125. doi:10.1016/j.fitote.2007.11.002 |
| spellingShingle | Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression |
| summary | Objective: Transforming growth factor-β (TGF-β) plays an important role in wound healing. Delayed wound healing is a consequence of diabetes, leading to high morbidity and poor quality of life. Momordica charantia (MC) fruit possesses anti-diabetic and wound healing properties. This study aimed to explore the changes in TGF-β expression in diabetic wounds treated with topical MC fruit extract. Methods: Fifty-six male Sprague-Dawley rats were divided into a normal control group and five diabetic groups of ten rats each. Intravenous streptozotocin (50mg/kg) was given to induce diabetes in the diabetic groups. Full thickness excision wounds were created on the thoracodorsal region of the animals, and these wounds were then treated with vehicle, MC powder, MC ointment and povidone ointment or ointment base for ten days. Wound healing was determined by the rate of wound closure, total protein content and TGF-β expression in the wounds, and histological observation. Results: Diabetic groups showed delayed wound closure rates compared to the control group. The wound closure rate in the MC ointment group was significantly faster than that of the untreated diabetic group (p<0.05). The MC ointment group also showed intense TGF-β expression and a high level of total protein content. Conclusion: MC ointment has a promising potential for use as an alternative topical medication for diabetic wounds. This work has shown that it accelerates wound healing in diabetic rats, and it is suggested here that this occurs by enhancing TGF-β expression. Further work is recommended to explore this effect. Declaration of interest: There is no conflict of interest. |
| title | Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression |
| title_full | Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression |
| title_fullStr | Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression |
| title_full_unstemmed | Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression |
| title_short | Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression |
| title_sort | momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression |