Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 |
| date | 2014-09-29 14:47:27 |
| format | Restricted Document |
| id | 11291 |
| institution | UniSZA |
| internalnotes | Adeleye, A., Vivian, E., Rita, N., Stella, S. and Emmanuel, O. 2008. Antimicrobial susceptibility of potentially pathogenic halophilic Vibrio species isolated from seafoods in Lagos, Nigeria. African Journal of Biotechnology 7: 3791-3794. Bilung, L.M., Radu, S., Rani, A.B, Raha, A.R., Suhaimi, N., Michael, W.C.V.L., Gwendelynne, B.T. and Nishibuchi, M. 2005. Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR. FEMS Microbiology Letters 252: 85-88. Chao, G., Jiao, X., Zhou, X., Yang, Z., Huang, J., Pan, Z., Zhou, L. and Qian, X. 2009. Serodiversity, Pandemic O3:K6 Clone, Molecular typing and antibiotic susceptibility of foodborne and clinical Vibrio parahaemolyticus isolates in Jiangsu, China. Foodborne Pathogens and Disease 6: 1021-1028. Clinical and Laboratory Standards Institute. 2007. Performance standards for antimicrobial susceptibility testing; seventeenth informational supplement. CLSI document M100-S17. Clinical and Laboratory Standards Institute. 2005. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; proposed guideline. CLSI document M45-P. FAO/WHO. 2011. Risk assessment of Vibrio parahaemolyticus in seafood: Interpretative summary and Technical Report. Microbiological Risk Assessment Series 16: 193. Gwendelynne, B.T., Son, R., Nishibuchi, M., Raha, A.R., Suhaimi, N., Lesley, M. and Jurin, W.G. 2005. Characterization of Vibrio parahaemolyticus isolated from coastal seawater in Peninsular Malaysia. The Southeast Asian Journal of Tropical Medicine and Public Health 36: 940-945. Khan, A.W., Hossain, S.J. and Uddin, S.N. 2007. Isolation, identification and determination of antibiotics susceptibility of Vibrio parahaemolyticus from shrimps at Khulna Region of Bangladesh. Research Journal of Microbiology 2: 216-227. Kim, Y.B., Okuda, J., Matsumoto, C., Takahashi, N., Hashimoto, S. and Nishibuchi, M. 1999. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. Journal of Clinical Microbiology 37: 1173-1177. Krumperman, P.H. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Applied and Environmental Microbiology 46: 165-170. Nelapati, S., Nelapati, K. and Chinnam, B. K. 2012. Vibrio parahaemolyticus - An emerging foodborne pathogenA Review. Veterinary World 5: 48-62. Noorlis, A., Ghazali, F.M., Cheah, Y.K., Tuan Zainazor, T.C., Ponniah, J., Tunung, R., Tang, J.Y.H., Nishibuchi, M., Nakaguchi, Y. and Son, R. 2011. Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level. International Food Research Journal 18: 689– 695. Oralak, S., Nurl, A.B., Gopinath, B.N., Orapan, C., Apichai, S., Ladaporn, B., Sinn, A. and Carl, J.M. 2007. The dominance of pandemic serovars of Vibrio parahaemolyticus in expatriates and sporadic cases of diarrhoea in Thailand, and a new emergent serovar (O3: K46) with pandemic traits. Journal of Medical Microbiology 56: 608-613. Ottaviani, D., Bacchiocchi, I., Masini, L., Francesca, L., Carraturo, A., Giammarioli, M. and Sbaraglia, G. 2001. Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood. International Journal of Antimicrobial Agents 18: 135-140. Ray, B. and Bhunia, A. 2008. Fundamental Food Microbiology. Fourth edition ed. CRC Press. Sakazaki, R., Kaysner, C. and Carlos, A.J. 2006. Vibrio infections. In Riemann, H. P. and Cliver, D. O. (Eds) Foodborne infections and intoxications. United States of America: Elesevier Inc. Sudha, S., Divya, P.S., Francis, B. and Hatha, A.A.M. 2012. Prevalence and distribution of Vibrio parahaemolyticus in finfish from Cochin (South India). Veterinaria Italiana 48: 269-281. Tunung, R., Jeyaletchumi, P., Noorlis, A., Tang, Y.H., Sandra, A., Ghazali, F.M., Noranizan, M.A., Lesley, M.B., Haresh, K.K., Nakaguchi, Y., Nishibuchi, M. and Son, R. 2012. Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling. International Food Research Journal 19: 467-474. Vengadesh, L., Son, R. and Yoke-Kqueen, C. 2012. Molecular Quantitation and Characterization of Vibrio cholerae From Different Seafood Obtained from Wetmarket and Supermarket. International Food Research Journal 19: 45-50. Zulkifli, Y., Alitheen, N.B., Son, R., Yeap, S.K., Lesley, M.B. and Raha, A.R. 2009. Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes. International Food Research Journal 16: 289-296. |
| originalfilename | 5505-01-FH02-FPBSM-06-01521.jpg |
| person | UniSZA Unisza unisza |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11291 |
| spelling | 11291 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11291 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 1427 33 33 2014-09-29 14:47:27 781 1427x781 5505-01-FH02-FPBSM-06-01521.jpg UniSZA Private Access Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia International Food Research Journal This study aimed to investigate the prevalence and antibiogram of Vibrio parahaemolyticus in processed bivalve molluscs in Kuala Terengganu. A total of 80 seafood samples, namely mussels (n=20), carpet clams (n=20), cockles (n=20) and scallops (n=20), were subjected to PCR and conventional plating method for the detection of V. parahaemolyticus. V. parahaemolyticus was found in green mussels (55%), carpet clam (80%), cockles (40%) and scallops (55%). Fiftyfive V. parahaemolyticus isolates were subjected to 9 types antibiotic sensitivity test using discs diffusion method. All isolates were susceptible to Tetracycline and Gentamycin. Isolates showed high resistance towards Vancomycin (52.73%), Penicillin (45.45%) and Amplicillin (32.73%). Resistance towards Amikacin, Ciprofloxacin and Norfloxacin were found to be 1.82%. It can be concluded that local bivalve molluscs were contaminated with V. parahaemolyticus and isolates showed resistance towards certain antibiotics. Therefore, consumption of raw or semicooked bivalve molluscs is not advisable. 21 4 1349-1353 Adeleye, A., Vivian, E., Rita, N., Stella, S. and Emmanuel, O. 2008. Antimicrobial susceptibility of potentially pathogenic halophilic Vibrio species isolated from seafoods in Lagos, Nigeria. African Journal of Biotechnology 7: 3791-3794. Bilung, L.M., Radu, S., Rani, A.B, Raha, A.R., Suhaimi, N., Michael, W.C.V.L., Gwendelynne, B.T. and Nishibuchi, M. 2005. Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR. FEMS Microbiology Letters 252: 85-88. Chao, G., Jiao, X., Zhou, X., Yang, Z., Huang, J., Pan, Z., Zhou, L. and Qian, X. 2009. Serodiversity, Pandemic O3:K6 Clone, Molecular typing and antibiotic susceptibility of foodborne and clinical Vibrio parahaemolyticus isolates in Jiangsu, China. Foodborne Pathogens and Disease 6: 1021-1028. Clinical and Laboratory Standards Institute. 2007. Performance standards for antimicrobial susceptibility testing; seventeenth informational supplement. CLSI document M100-S17. Clinical and Laboratory Standards Institute. 2005. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; proposed guideline. CLSI document M45-P. FAO/WHO. 2011. Risk assessment of Vibrio parahaemolyticus in seafood: Interpretative summary and Technical Report. Microbiological Risk Assessment Series 16: 193. Gwendelynne, B.T., Son, R., Nishibuchi, M., Raha, A.R., Suhaimi, N., Lesley, M. and Jurin, W.G. 2005. Characterization of Vibrio parahaemolyticus isolated from coastal seawater in Peninsular Malaysia. The Southeast Asian Journal of Tropical Medicine and Public Health 36: 940-945. Khan, A.W., Hossain, S.J. and Uddin, S.N. 2007. Isolation, identification and determination of antibiotics susceptibility of Vibrio parahaemolyticus from shrimps at Khulna Region of Bangladesh. Research Journal of Microbiology 2: 216-227. Kim, Y.B., Okuda, J., Matsumoto, C., Takahashi, N., Hashimoto, S. and Nishibuchi, M. 1999. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. Journal of Clinical Microbiology 37: 1173-1177. Krumperman, P.H. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Applied and Environmental Microbiology 46: 165-170. Nelapati, S., Nelapati, K. and Chinnam, B. K. 2012. Vibrio parahaemolyticus - An emerging foodborne pathogenA Review. Veterinary World 5: 48-62. Noorlis, A., Ghazali, F.M., Cheah, Y.K., Tuan Zainazor, T.C., Ponniah, J., Tunung, R., Tang, J.Y.H., Nishibuchi, M., Nakaguchi, Y. and Son, R. 2011. Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level. International Food Research Journal 18: 689– 695. Oralak, S., Nurl, A.B., Gopinath, B.N., Orapan, C., Apichai, S., Ladaporn, B., Sinn, A. and Carl, J.M. 2007. The dominance of pandemic serovars of Vibrio parahaemolyticus in expatriates and sporadic cases of diarrhoea in Thailand, and a new emergent serovar (O3: K46) with pandemic traits. Journal of Medical Microbiology 56: 608-613. Ottaviani, D., Bacchiocchi, I., Masini, L., Francesca, L., Carraturo, A., Giammarioli, M. and Sbaraglia, G. 2001. Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood. International Journal of Antimicrobial Agents 18: 135-140. Ray, B. and Bhunia, A. 2008. Fundamental Food Microbiology. Fourth edition ed. CRC Press. Sakazaki, R., Kaysner, C. and Carlos, A.J. 2006. Vibrio infections. In Riemann, H. P. and Cliver, D. O. (Eds) Foodborne infections and intoxications. United States of America: Elesevier Inc. Sudha, S., Divya, P.S., Francis, B. and Hatha, A.A.M. 2012. Prevalence and distribution of Vibrio parahaemolyticus in finfish from Cochin (South India). Veterinaria Italiana 48: 269-281. Tunung, R., Jeyaletchumi, P., Noorlis, A., Tang, Y.H., Sandra, A., Ghazali, F.M., Noranizan, M.A., Lesley, M.B., Haresh, K.K., Nakaguchi, Y., Nishibuchi, M. and Son, R. 2012. Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling. International Food Research Journal 19: 467-474. Vengadesh, L., Son, R. and Yoke-Kqueen, C. 2012. Molecular Quantitation and Characterization of Vibrio cholerae From Different Seafood Obtained from Wetmarket and Supermarket. International Food Research Journal 19: 45-50. Zulkifli, Y., Alitheen, N.B., Son, R., Yeap, S.K., Lesley, M.B. and Raha, A.R. 2009. Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes. International Food Research Journal 16: 289-296. |
| spellingShingle | Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia |
| summary | This study aimed to investigate the prevalence and antibiogram of Vibrio parahaemolyticus in processed bivalve molluscs in Kuala Terengganu. A total of 80 seafood samples, namely mussels (n=20), carpet clams (n=20), cockles (n=20) and scallops (n=20), were subjected to PCR and conventional plating method for the detection of V. parahaemolyticus. V. parahaemolyticus was found in green mussels (55%), carpet clam (80%), cockles (40%) and scallops (55%). Fiftyfive V. parahaemolyticus isolates were subjected to 9 types antibiotic sensitivity test using discs diffusion method. All isolates were susceptible to Tetracycline and Gentamycin. Isolates showed high resistance towards Vancomycin (52.73%), Penicillin (45.45%) and Amplicillin (32.73%). Resistance towards Amikacin, Ciprofloxacin and Norfloxacin were found to be 1.82%. It can be concluded that local bivalve molluscs were contaminated with V. parahaemolyticus and isolates showed resistance towards certain antibiotics. Therefore, consumption of raw or semicooked bivalve molluscs is not advisable. |
| title | Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia |
| title_full | Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia |
| title_fullStr | Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia |
| title_full_unstemmed | Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia |
| title_short | Incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia |
| title_sort | incidence and antibiogram of vibrio parahaemolyticus in processed and frozen bivalve mollusks in kuala terengganu, malaysia |