Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms

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date 2017-08-02 15:05:38
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originalfilename 5367-01-FH02-FP-17-10642.pdf
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spelling 11165 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11165 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 3 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 74 Azzubaidi 2017-08-02 15:05:38 5367-01-FH02-FP-17-10642.pdf UniSZA Private Access Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms International Journal of Pharmacy and Pharmaceutical Sciences Objective: The objectives of the current study were to confirm the blood pressure lowering effect of thymoquinone (TQ) and to investigate its mechanism through muscarinic and β-adrenergic receptors. Methods: Mean arterial blood pressure (MAP) was recorded using the non-invasive blood pressure tail-cuff technique. A dose-response relationship was obtained after using 3 TQ doses (2.5, 5 and 10 mg/kg) intraperitoneally to 3 different groups (n =5) of adult rats under pentobarbital anesthesia. MAP was then measured for another 2 animal groups pretreated either with atropine (P-at) or propranolol (P-pro) followed by 10 mg/kg TQ. Results: TQ produced a significant dose-dependent blood pressure and heart rate lowering effect. TQ-induced MAP reduction was significantly less pronounced in P-at (12±2.8 mmHg) than non-pretreated group (29±3.2 mmHg) with P<0.01. Conversely, TQ-induced MAP reduction in P-pro (28±3.4 mmHg) did not demonstrate a significant difference from the non-pretreated group (29±3.2 mmHg) with P>0.05. Conclusion: This study confirms the dose-related hypotensive effect of TQ and provides an evidence for the traditional use of Nigella sativa for the treatment of hypertension. The mechanism of TQ-induced hypotension involves at least in part activation of vascular muscarinic receptors, but not β-adrenergic receptors. 9 8 216-218
spellingShingle Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms
subject 74
summary Objective: The objectives of the current study were to confirm the blood pressure lowering effect of thymoquinone (TQ) and to investigate its mechanism through muscarinic and β-adrenergic receptors. Methods: Mean arterial blood pressure (MAP) was recorded using the non-invasive blood pressure tail-cuff technique. A dose-response relationship was obtained after using 3 TQ doses (2.5, 5 and 10 mg/kg) intraperitoneally to 3 different groups (n =5) of adult rats under pentobarbital anesthesia. MAP was then measured for another 2 animal groups pretreated either with atropine (P-at) or propranolol (P-pro) followed by 10 mg/kg TQ. Results: TQ produced a significant dose-dependent blood pressure and heart rate lowering effect. TQ-induced MAP reduction was significantly less pronounced in P-at (12±2.8 mmHg) than non-pretreated group (29±3.2 mmHg) with P<0.01. Conversely, TQ-induced MAP reduction in P-pro (28±3.4 mmHg) did not demonstrate a significant difference from the non-pretreated group (29±3.2 mmHg) with P>0.05. Conclusion: This study confirms the dose-related hypotensive effect of TQ and provides an evidence for the traditional use of Nigella sativa for the treatment of hypertension. The mechanism of TQ-induced hypotension involves at least in part activation of vascular muscarinic receptors, but not β-adrenergic receptors.
title Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms
title_full Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms
title_fullStr Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms
title_full_unstemmed Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms
title_short Hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms
title_sort hypotensive activity of thymoquinone in normotensive rats and its receptor mechanisms