Morphological and phenotypical characteristics of human osteoblasts after short-term space mission
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 |
| date | 2014-07-02 14:09:10 |
| format | Restricted Document |
| id | 11110 |
| institution | UniSZA |
| internalnotes | 1. Buravkova, L. B., Gershovich, P. M., Gershovich, J. G., & Grigor'ev, A. I. (2010). Mechanisms of gravitational sensitivity of osteogenic precursor cells. Acta Naturae, 2(1), 28-36. Retrieved from www.scopus.com 2. Gershovich, P. M., Gershovich, J. G., & Buravkova, L. B. (2009). Cytoskeleton structures and adhesion properties of human stromal precursors inder conditions of simulated microgravity. Tsitologiya, 51(11), 896-903. Retrieved from www.scopus.com 3. Hughes-Fulford, M., Rodenacker, K., & Jütting, U. (2006). Reduction of anabolic signals and alteration of osteoblast nuclear morphology in microgravity. Journal of Cellular Biochemistry, 99(2), 435-449. doi:10.1002/jcb.20883 4. Kapitonova, M. Y., Kuznetsov, S. L., Froemming, G. R. A., Muid, S., Nor-Ashikin, M. N. K., Otman, S., . . . Nawawi, H. (2013). Effects of space mission factors on the morphology and function of endothelial cells. Bulletin of Experimental Biology and Medicine, 154(6), 796-801. doi:10.1007/s10517-013-2059-7 5. Kumei, Y., Morita, S., Katano, H., Akiyama, H., Hirano, M., Oyha, K., & Shimokawa, H. (2006). Microgravity signal ensnarls cell adhesion, cytoskeleton, and matrix proteins of rat osteoblasts: Osteopontin, CD44, osteonectin, and α-tubulin doi:10.1196/annals.1378.034 Retrieved from www.scopus.com 6. Meyers, V. E., Zayzafoon, M., Douglas, J. T., & McDonald, J. M. (2005). RhoA and cytoskeletal disruption mediate reduced osteoblastogenesis and enhanced adipogenesis of human mesenchymal stem cells in modeled microgravity. Journal of Bone and Mineral Research, 20(10), 1858-1866. doi:10.1359/JBMR.050611 7. Monticone, M., Liu, Y., Pujic, N., & Cancedda, R. (2010). Activation of nervous system development genes in bone marrow derived mesenchymal stem cells following spaceflight exposure. Journal of Cellular Biochemistry, 111(2), 442-452. doi:10.1002/jcb.22765 8. Nabavi, N., Khandani, A., Camirand, A., & Harrison, R. E. (2011). Effects of microgravity on osteoclast bone resorption and osteoblast cytoskeletal organization and adhesion. Bone, 49(5), 965-974. doi:10.1016/j.bone.2011.07.036 9. Nagaraja, M. P., & Risin, D. (2013). The current state of bone loss research: Data from spaceflight and microgravity simulators. Journal of Cellular Biochemistry, 114(5), 1001-1008. doi:10.1002/jcb.24454 10. Oganov, V. S., & Grigor'ev, A. I. (2012). [Mechanisms of human osteopenia and some peculiarities of bone metabolism in weightlessness conditions]. Rossiǐskii Fiziologicheskiǐ Zhurnal Imeni I.M.Sechenova / Rossiǐskaia Akademiia Nauk, 98(3), 395-409. Retrieved from www.scopus.com 11. Qian, A. R., Li, D., Han, J., Gao, X., Di, S. M., Zhang, W., . . . Shang, P. (2012). Fractal dimension as a measure of altered actin cytoskeleton in MC3T3-E1 cells under simulated microgravity using 3-D/2-D clinostats. IEEE Transactions on Biomedical Engineering, 59(5), 1374-1380. doi:10.1109/TBME.2012.2187785 12. Saravia, F., Núñez-Martínez, I., Morán, J. M., Soler, C., Muriel, A., Rodríguez-Martínez, H., & Peña, F. J. (2007). Differences in boar sperm head shape and dimensions recorded by computer-assisted sperm morphometry are not related to chromatin integrity. Theriogenology, 68(2), 196-203. doi:10.1016/j.theriogenology.2007.04.052 13. Schatten, H., Lewis, M. L., & Chakrabarti, A. (2001). Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells. Acta Astronautica, 49(3-10), 399-418. doi:10.1016/S0094-5765(01)00116-3 14. Tamma, R., Colaianni, G., Camerino, C., Di Benedetto, A., Greco, G., Strippoli, M., . . . Zallone, A. (2009). Microgravity during spaceflight directly affects in vitro osteoclastogenesis and bone resorption. FASEB Journal, 23(8), 2549-2554. doi:10.1096/fj.08-127951 15. Vico, L. (2006). What do we know about alteration in the osteoblast phenotype with microgravity? Journal of Musculoskeletal Neuronal Interactions, 6(4), 317-318. Retrieved from www.scopus.com |
| originalfilename | 5307-01-FH02-FPBSM-14-00782.jpg |
| person | UniSZA Unisza unisza |
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| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11110 |
| spelling | 11110 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=11110 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 1421 13 13 786 2014-07-02 14:09:10 1421x786 5307-01-FH02-FPBSM-14-00782.jpg UniSZA Private Access Morphological and phenotypical characteristics of human osteoblasts after short-term space mission Bulletin of Experimental Biology and Medicine Morphological and phenotypical signs of cultured readaptation osteoblasts were studied after a short-term space mission. The ultrastructure and phenotype of human osteoblasts after Soyuz TMA-11 space flight (2007) were evaluated by scanning electron microscopy, laser confocal microscopy, and ELISA. The morphofunctional changes in cell cultures persisted after 12 passages. Osteoblasts retained the drastic changes in their shape and size, contour deformation, disorganization of the microtubular network, redistribution of organelles and specialized structures of the plasmalemma in comparison with the ground control cells. On the other hand, the expression of osteoprotegerin and osteocalcin (bone metabolism markers) increased; the expression of bone resorption markers ICAM-1 and IL-6 also increased, while the expression of VCAM-1 decreased. Hence, space flight led to the development of persistent shifts in cultured osteoblasts indicating injuries to the cytoskeleton and the phenotype changes, indicating modulation of bone metabolism biomarkers. 156 3 393-398 1. Buravkova, L. B., Gershovich, P. M., Gershovich, J. G., & Grigor'ev, A. I. (2010). Mechanisms of gravitational sensitivity of osteogenic precursor cells. Acta Naturae, 2(1), 28-36. Retrieved from www.scopus.com 2. Gershovich, P. M., Gershovich, J. G., & Buravkova, L. B. (2009). Cytoskeleton structures and adhesion properties of human stromal precursors inder conditions of simulated microgravity. Tsitologiya, 51(11), 896-903. Retrieved from www.scopus.com 3. Hughes-Fulford, M., Rodenacker, K., & Jütting, U. (2006). Reduction of anabolic signals and alteration of osteoblast nuclear morphology in microgravity. Journal of Cellular Biochemistry, 99(2), 435-449. doi:10.1002/jcb.20883 4. Kapitonova, M. Y., Kuznetsov, S. L., Froemming, G. R. A., Muid, S., Nor-Ashikin, M. N. K., Otman, S., . . . Nawawi, H. (2013). Effects of space mission factors on the morphology and function of endothelial cells. Bulletin of Experimental Biology and Medicine, 154(6), 796-801. doi:10.1007/s10517-013-2059-7 5. Kumei, Y., Morita, S., Katano, H., Akiyama, H., Hirano, M., Oyha, K., & Shimokawa, H. (2006). Microgravity signal ensnarls cell adhesion, cytoskeleton, and matrix proteins of rat osteoblasts: Osteopontin, CD44, osteonectin, and α-tubulin doi:10.1196/annals.1378.034 Retrieved from www.scopus.com 6. Meyers, V. E., Zayzafoon, M., Douglas, J. T., & McDonald, J. M. (2005). RhoA and cytoskeletal disruption mediate reduced osteoblastogenesis and enhanced adipogenesis of human mesenchymal stem cells in modeled microgravity. Journal of Bone and Mineral Research, 20(10), 1858-1866. doi:10.1359/JBMR.050611 7. Monticone, M., Liu, Y., Pujic, N., & Cancedda, R. (2010). Activation of nervous system development genes in bone marrow derived mesenchymal stem cells following spaceflight exposure. Journal of Cellular Biochemistry, 111(2), 442-452. doi:10.1002/jcb.22765 8. Nabavi, N., Khandani, A., Camirand, A., & Harrison, R. E. (2011). Effects of microgravity on osteoclast bone resorption and osteoblast cytoskeletal organization and adhesion. Bone, 49(5), 965-974. doi:10.1016/j.bone.2011.07.036 9. Nagaraja, M. P., & Risin, D. (2013). The current state of bone loss research: Data from spaceflight and microgravity simulators. Journal of Cellular Biochemistry, 114(5), 1001-1008. doi:10.1002/jcb.24454 10. Oganov, V. S., & Grigor'ev, A. I. (2012). [Mechanisms of human osteopenia and some peculiarities of bone metabolism in weightlessness conditions]. Rossiǐskii Fiziologicheskiǐ Zhurnal Imeni I.M.Sechenova / Rossiǐskaia Akademiia Nauk, 98(3), 395-409. Retrieved from www.scopus.com 11. Qian, A. R., Li, D., Han, J., Gao, X., Di, S. M., Zhang, W., . . . Shang, P. (2012). Fractal dimension as a measure of altered actin cytoskeleton in MC3T3-E1 cells under simulated microgravity using 3-D/2-D clinostats. IEEE Transactions on Biomedical Engineering, 59(5), 1374-1380. doi:10.1109/TBME.2012.2187785 12. Saravia, F., Núñez-Martínez, I., Morán, J. M., Soler, C., Muriel, A., Rodríguez-Martínez, H., & Peña, F. J. (2007). Differences in boar sperm head shape and dimensions recorded by computer-assisted sperm morphometry are not related to chromatin integrity. Theriogenology, 68(2), 196-203. doi:10.1016/j.theriogenology.2007.04.052 13. Schatten, H., Lewis, M. L., & Chakrabarti, A. (2001). Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells. Acta Astronautica, 49(3-10), 399-418. doi:10.1016/S0094-5765(01)00116-3 14. Tamma, R., Colaianni, G., Camerino, C., Di Benedetto, A., Greco, G., Strippoli, M., . . . Zallone, A. (2009). Microgravity during spaceflight directly affects in vitro osteoclastogenesis and bone resorption. FASEB Journal, 23(8), 2549-2554. doi:10.1096/fj.08-127951 15. Vico, L. (2006). What do we know about alteration in the osteoblast phenotype with microgravity? Journal of Musculoskeletal Neuronal Interactions, 6(4), 317-318. Retrieved from www.scopus.com |
| spellingShingle | Morphological and phenotypical characteristics of human osteoblasts after short-term space mission |
| summary | Morphological and phenotypical signs of cultured readaptation osteoblasts were studied after a short-term space mission. The ultrastructure and phenotype of human osteoblasts after Soyuz TMA-11 space flight (2007) were evaluated by scanning electron microscopy, laser confocal microscopy, and ELISA. The morphofunctional changes in cell cultures persisted after 12 passages. Osteoblasts retained the drastic changes in their shape and size, contour deformation, disorganization of the microtubular network, redistribution of organelles and specialized structures of the plasmalemma in comparison with the ground control cells. On the other hand, the expression of osteoprotegerin and osteocalcin (bone metabolism markers) increased; the expression of bone resorption markers ICAM-1 and IL-6 also increased, while the expression of VCAM-1 decreased. Hence, space flight led to the development of persistent shifts in cultured osteoblasts indicating injuries to the cytoskeleton and the phenotype changes, indicating modulation of bone metabolism biomarkers. |
| title | Morphological and phenotypical characteristics of human osteoblasts after short-term space mission |
| title_full | Morphological and phenotypical characteristics of human osteoblasts after short-term space mission |
| title_fullStr | Morphological and phenotypical characteristics of human osteoblasts after short-term space mission |
| title_full_unstemmed | Morphological and phenotypical characteristics of human osteoblasts after short-term space mission |
| title_short | Morphological and phenotypical characteristics of human osteoblasts after short-term space mission |
| title_sort | morphological and phenotypical characteristics of human osteoblasts after short-term space mission |