Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
| Format: | Restricted Document |
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| _version_ | 1860796983200972800 |
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| building | INTELEK Repository |
| caption | Asian Pacific Journal of Tropical Biomedicine, 6 (2016) 71-75. doi:10.1016/j.apjtb.2015.10.002 |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 |
| copyright | © 2015 Hainan Medical University. Production and hosting by Elsevier (Singapore) Pte Ltd. |
| date | 18th December 2015 |
| format | Restricted Document |
| id | 10927 |
| institution | UniSZA |
| originalfilename | 5071-01-FH02-FBIM-16-05225.pdf |
| person | John Yew Huat Tang Mohd Ikhsan Khalid Syazana Aimi Che Abdullah Abu-Bakar Son Radu |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=10927 |
| spelling | 10927 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=10927 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 5 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 1.7 John Yew Huat Tang Mohd Ikhsan Khalid Syazana Aimi Che Abdullah Abu-Bakar Son Radu © 2015 Hainan Medical University. Production and hosting by Elsevier (Singapore) Pte Ltd. 18th December 2015 Asian Pacific Journal of Tropical Biomedicine, 6 (2016) 71-75. doi:10.1016/j.apjtb.2015.10.002 Campylobacter jejuni Multiple antibiotic resistance index Antibiotic resistance RAPD analysis Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets 5071-01-FH02-FBIM-16-05225.pdf Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets UniSZA Private Access Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets Asian Pacific Journal of Tropical Biomedicine Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates. Results: Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), gentamycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance towards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets. Conclusions: C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse. 6 1 71-75 |
| spellingShingle | Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets |
| subject | Campylobacter jejuni Multiple antibiotic resistance index Antibiotic resistance RAPD analysis |
| summary | Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates. Results: Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), gentamycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance towards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets. Conclusions: C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse. |
| title | Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets |
| title_full | Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets |
| title_fullStr | Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets |
| title_full_unstemmed | Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets |
| title_short | Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets |
| title_sort | antibiotic resistance profile and rapd analysis of campylobacter jejuni isolated from vegetables farms and retail markets |