Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo
Cas9, a CRISPR-associated RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although complexes between Cas9 and guide RNAs (gRNAs) offer remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the und...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914125/ |