Identification of four new gene members of the KAP6 gene family in sheep

KAP6 is a high glycine-tyrosine keratin-associated protein (HGT-KAP) family. This family is thought to contain multiple genes. In this study, we used a KRTAP6 coding sequence to search the Ovine Genome (v3.1) and identified five homologous regions (R1–R5). All these regions contained an open reading...

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Main Authors:	Zhou, Huitong, Gong, Hua, Wang, Jiqing, Dyer, Jolon M., Luo, Yuzhu, Hickford, Jon G. H.
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820716/

id	pubmed-4820716
recordtype	oai_dc
spelling	pubmed-48207162016-04-06 Identification of four new gene members of the KAP6 gene family in sheep Zhou, Huitong Gong, Hua Wang, Jiqing Dyer, Jolon M. Luo, Yuzhu Hickford, Jon G. H. Article KAP6 is a high glycine-tyrosine keratin-associated protein (HGT-KAP) family. This family is thought to contain multiple genes. In this study, we used a KRTAP6 coding sequence to search the Ovine Genome (v3.1) and identified five homologous regions (R1–R5). All these regions contained an open reading frame, and they were either identical to, or highly similar to, sheep skin Expressed Sequence Tags (ESTs). Phylogenetic analysis revealed that R1–R5 were clustered with KAP6 sequences from different species and formed a group distinct to other HGT-KAPs. R1 was very similar to the characterised KRTAP6-1 sequence, but the remaining genes appeared to be new. PCR primers were designed to amplify and confirm the presence of these new genes. Amplicons were obtained for all of the 96 sheep investigated. Six, five, three and six PCR-SSCP patterns representing six, five, three and six DNA sequences were observed for KRTAP6-2 to KRTAP6-5 respectively. KRTAP6-2 and KRTAP6-4 had five and three SNPs respectively. Three SNPs and a 45-bp insertion/deletion were detected for KRTAP6-3, and five SNPs and an 18-bp insertion/deletion were identified for KRTAP6-5. Allele frequencies for these KAP6 genes differed between Merino and Romney sheep. Nature Publishing Group 2016-04-05 /pmc/articles/PMC4820716/ /pubmed/27045687 http://dx.doi.org/10.1038/srep24074 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhou, Huitong Gong, Hua Wang, Jiqing Dyer, Jolon M. Luo, Yuzhu Hickford, Jon G. H.
spellingShingle	Zhou, Huitong Gong, Hua Wang, Jiqing Dyer, Jolon M. Luo, Yuzhu Hickford, Jon G. H. Identification of four new gene members of the KAP6 gene family in sheep
author_facet	Zhou, Huitong Gong, Hua Wang, Jiqing Dyer, Jolon M. Luo, Yuzhu Hickford, Jon G. H.
author_sort	Zhou, Huitong
title	Identification of four new gene members of the KAP6 gene family in sheep
title_short	Identification of four new gene members of the KAP6 gene family in sheep
title_full	Identification of four new gene members of the KAP6 gene family in sheep
title_fullStr	Identification of four new gene members of the KAP6 gene family in sheep
title_full_unstemmed	Identification of four new gene members of the KAP6 gene family in sheep
title_sort	identification of four new gene members of the kap6 gene family in sheep
description	KAP6 is a high glycine-tyrosine keratin-associated protein (HGT-KAP) family. This family is thought to contain multiple genes. In this study, we used a KRTAP6 coding sequence to search the Ovine Genome (v3.1) and identified five homologous regions (R1–R5). All these regions contained an open reading frame, and they were either identical to, or highly similar to, sheep skin Expressed Sequence Tags (ESTs). Phylogenetic analysis revealed that R1–R5 were clustered with KAP6 sequences from different species and formed a group distinct to other HGT-KAPs. R1 was very similar to the characterised KRTAP6-1 sequence, but the remaining genes appeared to be new. PCR primers were designed to amplify and confirm the presence of these new genes. Amplicons were obtained for all of the 96 sheep investigated. Six, five, three and six PCR-SSCP patterns representing six, five, three and six DNA sequences were observed for KRTAP6-2 to KRTAP6-5 respectively. KRTAP6-2 and KRTAP6-4 had five and three SNPs respectively. Three SNPs and a 45-bp insertion/deletion were detected for KRTAP6-3, and five SNPs and an 18-bp insertion/deletion were identified for KRTAP6-5. Allele frequencies for these KAP6 genes differed between Merino and Romney sheep.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820716/
_version_	1613562211254403072

Identification of four new gene members of the KAP6 gene family in sheep

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