Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function
Dendritic and axonal arbors of many neuronal types exhibit self-avoidance, in which branches repel each other. In some cases, these neurites interact with those of neighboring neurons, a phenomenon called self/non-self discrimination. The functional roles of these processes remain unknown. In this s...
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| Format: | Online |
| Language: | English |
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eLife Sciences Publications, Ltd
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548410/ |
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pubmed-4548410 |
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pubmed-45484102015-08-26 Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function Kostadinov, Dimitar Sanes, Joshua R Developmental Biology and Stem Cells Dendritic and axonal arbors of many neuronal types exhibit self-avoidance, in which branches repel each other. In some cases, these neurites interact with those of neighboring neurons, a phenomenon called self/non-self discrimination. The functional roles of these processes remain unknown. In this study, we used retinal starburst amacrine cells (SACs), critical components of a direction-selective circuit, to address this issue. In SACs, both processes are mediated by the gamma-protocadherins (Pcdhgs), a family of 22 recognition molecules. We manipulated Pcdhg expression in SACs and recorded from them and their targets, direction-selective ganglion cells (DSGCs). SACs form autapses when self-avoidance is disrupted and fail to form connections with other SACs when self/non-self discrimination is perturbed. Pcdhgs are also required to prune connections between closely spaced SACs. These alterations degrade the direction selectivity of DSGCs. Thus, self-avoidance, self/non-self discrimination, and synapse elimination are essential for proper function of a circuit that computes directional motion. eLife Sciences Publications, Ltd 2015-07-03 /pmc/articles/PMC4548410/ /pubmed/26140686 http://dx.doi.org/10.7554/eLife.08964 Text en © 2015, Kostadinov and Sanes http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Kostadinov, Dimitar Sanes, Joshua R |
| spellingShingle |
Kostadinov, Dimitar Sanes, Joshua R Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| author_facet |
Kostadinov, Dimitar Sanes, Joshua R |
| author_sort |
Kostadinov, Dimitar |
| title |
Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_short |
Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_full |
Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_fullStr |
Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_full_unstemmed |
Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_sort |
protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| description |
Dendritic and axonal arbors of many neuronal types exhibit self-avoidance, in which branches repel each other. In some cases, these neurites interact with those of neighboring neurons, a phenomenon called self/non-self discrimination. The functional roles of these processes remain unknown. In this study, we used retinal starburst amacrine cells (SACs), critical components of a direction-selective circuit, to address this issue. In SACs, both processes are mediated by the gamma-protocadherins (Pcdhgs), a family of 22 recognition molecules. We manipulated Pcdhg expression in SACs and recorded from them and their targets, direction-selective ganglion cells (DSGCs). SACs form autapses when self-avoidance is disrupted and fail to form connections with other SACs when self/non-self discrimination is perturbed. Pcdhgs are also required to prune connections between closely spaced SACs. These alterations degrade the direction selectivity of DSGCs. Thus, self-avoidance, self/non-self discrimination, and synapse elimination are essential for proper function of a circuit that computes directional motion. |
| publisher |
eLife Sciences Publications, Ltd |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548410/ |
| _version_ |
1613263404889276416 |