Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?

Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?

The genomic nucleotide sequences of japonica rice (Sasanishiki and Nipponbare) contained about 2.7-kb unique region at the point of 0.4-kb upstream of the OsPsbS1 gene. In this study, we found that japonica rice with a few exceptions possessing such DNA sequences [denoted to OsMULE-japonica specific...

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Main Authors: Nuruzzaman, Mohammed, Kanno, Tatsuo, Amada, Rika, Habu, Yoshiki, Kasajima, Ichiro, Ishikawa, Toshiki, Kawai-Yamada, Maki, Uchimiya, Hirofumi
Format: Online
Language:English
Published: Public Library of Science 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178011/
id pubmed-4178011
recordtype oai_dc
spelling pubmed-41780112014-10-02 Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression? Nuruzzaman, Mohammed Kanno, Tatsuo Amada, Rika Habu, Yoshiki Kasajima, Ichiro Ishikawa, Toshiki Kawai-Yamada, Maki Uchimiya, Hirofumi Research Article The genomic nucleotide sequences of japonica rice (Sasanishiki and Nipponbare) contained about 2.7-kb unique region at the point of 0.4-kb upstream of the OsPsbS1 gene. In this study, we found that japonica rice with a few exceptions possessing such DNA sequences [denoted to OsMULE-japonica specific sequence (JSS)] is distinct by the presence of Mutator-like-element (MULE). Such sequence was absent in most of indica cultivars and Oryza glaberrima. In OsMULE-JSS1, we noted the presence of possible target site duplication (TSD; CTTTTCCAG) and about 80-bp terminal inverted repeat (TIR) near TSD. We also found the enhancement ofOsPsbS1 mRNA accumulation by intensified light, which was not associated with the DNA methylation status in OsMULE/JSS. In addition, O. rufipogon, possible ancestor of modern rice cultivars was found to compose PsbS gene of either japonica (minor) or indica (major) type. Transient gene expression assay showed that the japonica type promoter elevated a reporter gene activity than indica type. Public Library of Science 2014-09-26 /pmc/articles/PMC4178011/ /pubmed/25259844 http://dx.doi.org/10.1371/journal.pone.0102742 Text en © 2014 Nuruzzaman et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Nuruzzaman, Mohammed
Kanno, Tatsuo
Amada, Rika
Habu, Yoshiki
Kasajima, Ichiro
Ishikawa, Toshiki
Kawai-Yamada, Maki
Uchimiya, Hirofumi
spellingShingle Nuruzzaman, Mohammed
Kanno, Tatsuo
Amada, Rika
Habu, Yoshiki
Kasajima, Ichiro
Ishikawa, Toshiki
Kawai-Yamada, Maki
Uchimiya, Hirofumi
Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?
author_facet Nuruzzaman, Mohammed
Kanno, Tatsuo
Amada, Rika
Habu, Yoshiki
Kasajima, Ichiro
Ishikawa, Toshiki
Kawai-Yamada, Maki
Uchimiya, Hirofumi
author_sort Nuruzzaman, Mohammed
title Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?
title_short Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?
title_full Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?
title_fullStr Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?
title_full_unstemmed Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?
title_sort does the upstream region possessing mule-like sequence in rice upregulate psbs1 gene expression?
description The genomic nucleotide sequences of japonica rice (Sasanishiki and Nipponbare) contained about 2.7-kb unique region at the point of 0.4-kb upstream of the OsPsbS1 gene. In this study, we found that japonica rice with a few exceptions possessing such DNA sequences [denoted to OsMULE-japonica specific sequence (JSS)] is distinct by the presence of Mutator-like-element (MULE). Such sequence was absent in most of indica cultivars and Oryza glaberrima. In OsMULE-JSS1, we noted the presence of possible target site duplication (TSD; CTTTTCCAG) and about 80-bp terminal inverted repeat (TIR) near TSD. We also found the enhancement ofOsPsbS1 mRNA accumulation by intensified light, which was not associated with the DNA methylation status in OsMULE/JSS. In addition, O. rufipogon, possible ancestor of modern rice cultivars was found to compose PsbS gene of either japonica (minor) or indica (major) type. Transient gene expression assay showed that the japonica type promoter elevated a reporter gene activity than indica type.
publisher Public Library of Science
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178011/
_version_ 1613138354916818944

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