Generation of Antibody-Producing Hybridomas Following One Single Immunization with a Targeted DNA Vaccine
The standard protocol for generating antibody (Ab)-producing hybridomas is based on fusion of plasmacytoma cells with Ab-producing B cells harvested from immunized mice. To increase the yield of hybridomas, it is important to use immunization protocols that induce a high frequency of B cells produci...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Blackwell Publishing Ltd
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417379/ |